Wu, G.; Wu, X.; Wu, Y.; Wu, Y.; Li, H.; Dai, C. Comprehensive transcriptome profiling of T-2 toxin-induced nephrotoxicity in mice. Preprints2023, 2023120727. https://doi.org/10.20944/preprints202312.0727.v1
APA Style
Wu, G., Wu, X., Wu, Y., Wu, Y., Li, H., & Dai, C. (2023). Comprehensive transcriptome profiling of T-2 toxin-induced nephrotoxicity in mice. Preprints. https://doi.org/10.20944/preprints202312.0727.v1
Chicago/Turabian Style
Wu, G., Hui Li and Chongshan Dai. 2023 "Comprehensive transcriptome profiling of T-2 toxin-induced nephrotoxicity in mice" Preprints. https://doi.org/10.20944/preprints202312.0727.v1
Abstract
Trichothecene mycotoxin T-2 toxin is an important environmental pollutant and poses a global threat to human and animal health. T-2 toxin could induce nephrotoxicity, however, the precise molecular mechanism remains unclear. In this study, the mice were intraperitoneally administrated with a single dose of 2 mg/kg T-2 toxin. The kidney function and ultrastructural observation were also assessed and found T-2 toxin exposure could cause kidney damage. Transmission electron microscopy showed a significant swelling and vacuolization of the mitochondria of renal cell. A total of 1122, 58, and 391 differential expression genes (DEGs) that were predominantly expressed in kidney tissues at 1 d, 3 d, and 7 d after T-2 toxin exposure, respectively. Early transcriptomic changes at 1 d after exposure were down-regulation of DEGs involved in the cell cycle, p53 signaling pathway, and cellular senescence, while up-regulation of DEGs referred to the ribosome pathway. The temporal variations in gene expression pattern in T-2 toxin exposure in mice kidney were presented and cellular metabolism was disturbed at the exposure recovery period with 7 d. In conclusion, this study, for the first time, provided a comprehensive comparative transcriptomic analysis of gene regulation in T-2 toxin exposure-induced nephrotoxicity at different temporal periods and explored the nephrotoxicity mechanism of T-2 toxin at the mRNA level.
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