Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza a Virus

Natalya Eshchenko
,
Mariia Sergeeva
ORCID logo ,
Evgenii Zhuravlev
* ORCID logo ,
Kira Kudria
,
Elena Goncharova
,
Andrey Komissarov
ORCID logo ,
Grigory Stepanov
ORCID logo
Version 1 : Received: 8 December 2023 / Approved: 8 December 2023 / Online: 8 December 2023 (12:42:54 CET)

A peer-reviewed article of this Preprint also exists.

Eshchenko, N.; Sergeeva, M.; Zhuravlev, E.; Kudria, K.; Goncharova, E.; Komissarov, A.; Stepanov, G. A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza A Virus. Int. J. Mol. Sci. 2024, 25, 625. Eshchenko, N.; Sergeeva, M.; Zhuravlev, E.; Kudria, K.; Goncharova, E.; Komissarov, A.; Stepanov, G. A Knockout of the IFITM3 Gene Increases the Sensitivity of WI-38 VA13 Cells to the Influenza A Virus. Int. J. Mol. Sci. 2024, 25, 625.

Abstract

One of the ways to regulate the sensitivity of human cells to the influenza virus is to knock out genes of the innate immune response. Promising targets for the knockout are genes of the interferon-inducible transmembrane protein (IFITM) family, in particular the IFITM3 gene, whose product limits the entry of a virus into the cell by blocking the fusion of the viral and endosomal membranes. In this study, by means of genome-editing system CRISPR/Cas9, monoclonal cell lines with an IFITM3 knockout were obtained based on WI-38 VA13 cells (human origin). It was found that such cell lines are more sensitive to infection by influenza A viruses of various subtypes. Nonetheless, this feature is not accompanied by an increased titer of newly formed viral particles in a culture medium.

Keywords

IFITM; Influenza A virus; Human cells; WI-38; Genome editing; CRISPR/Cas9; RNA-seq

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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