Effects of Dietary Supplementation with Tea Residue on Growth Performance, Digestibility, and Diarrhea in Piglets

1. Introduction

Early weaning of piglets is prone to diarrhea. Weaning stress leads to insufficient secretion of digestive enzymes in the gastrointestinal tract of piglets, which, together with the alkaline environment of the digestive tract itself, leads to easy infection of pathogenic bacteria and viruses in the intestines of piglets, resulting in diarrhea in weaned piglets [1]. The post-weaning period is characterized by a high incidence of intestinal disorders in piglets with diarrhea and growth performance inhibition [2]. Post-weaning diarrhea is a disease that causes great economic losses [3]. A study has found that post-weaning piglet mortality due to intestinal health problems ranges from 6% to 10% and sometimes up to 20% [4]. Imbalance of intestinal ion absorption and secretion is an important pathological mechanism of diarrhea, and many forms of diarrhea diseases depend on a related imbalance between the secretory and absorptive functions of the intestinal epithelium [5]. Diarrhea can be caused by increased secretion of osmotic active electrolytes (secretory diarrhea) or increased intake of osmotic active substances (osmotic diarrhea) [6]. When the osmotic pressure in the intestinal lumen is higher than in the intestinal wall, a large amount of water from the intestinal wall seeps into the intestinal lumen, causing the intestinal canal to dilate and peristalsis to accelerate, producing diarrhea [6], where the concentration of Na⁺ and K⁺ is the basis of osmotic diarrhea. Intestinal mucosal injury, such as inflammation and ulceration, leads to increased local vascular permeability and leakage of proteins and glucose into the intestine, resulting in increased osmotic pressure in the digestive tract and triggering diarrhea [7]. It is diarrhea that occurs when electrolytes with osmotic activity (e.g: Cl^-) are actively secreted into the intestinal lumen for various pathological reasons, leading to a passive influx of water into the intestinal lumen, increasing in the volume of the intestinal lumen contents and accelerating intestinal peristalsis [8].Therefore, regulation of intestinal ion transport in piglets may have an ameliorating effect on post weaning diarrhea.

Multiple beneficial components in tea (such as protein, tea polyphenols, polysaccharides, vitamins and minerals) promote animals health. Different bioactive components have a role in regulating various physiological and metabolic disorders [9]. Tea residue is rich in proteins up to 21-28 g/100g dry weight and has functional activities such as antioxidant, antimutagenic and hypolipidemic [10], and, therefore, a high nutritional value. J. Liang et al. used alkali extraction and acid precipitation method to extract tea residue protein from tea residue and found that tea residue protein enzymatic digestion products have good antioxidant capacity [11]. Studies have shown that green tea protein hydrolysates show antihypertensive activity [12]. Tea polyphenols have received attention from researchers and the public for their potential health functions, such as anticancer [13], antioxidant [14], hypoglycemic [15], anti-cardiovascular disease and antibacterial activities. Studies have shown that tea polyphenols can inhibit neuroinflammation and prevent degenerative effects in the brain and can be considered as potential neuroprotective substances [16]. It has been shown that the source and composition of fibrous carbohydrates in calf rations can influence the digestion and utilization of nutrients by calves [17]. When tea dietary fiber was prepared from tea residues and fed to mice on a high-fat diet, it was found to reduce total serum and liver cholesterol and decrease lipid peroxidation [18]. There is much evidence that herbs such as tea can be effective against diarrhea [19]. Studies suggest that green tea may be a possible complementary treatment for acute radiological diarrhea [20], the intake of a certain amount of green tea extract can improve diarrhea [21]. Fermented tea has been used for the treatment of pediatric diarrhea in Hunan and Sichuan regions of China since long ago, but the mechanism is not well understood.

This paper investigated the effects of fermented Fuzhuan tea residue on nutrient digestibility, growth performance, diarrhea condition, intestinal morphology and structure, ion channel expression level, biochemical and antioxidant indexes in plasma of weaned piglets. It was confirmed that tea residue can improve the diarrhea of weaned piglets, and the functional mechanism of anti-diarrhea of tea was preliminarily revealed by whole-cell patch clamp detection.

2. Materials and Methods

2.1. Experimental materials

Tea Residue

The tea residue is derived from the "Jinxiangyi" Fuzhuan tea produced by Yiyang Tea Factory in Hunan Province. The tea leaves are soaked in hot water at 80 °C for 5 min, discard the supernatant, collect the tea residue and drying, followed by crushing machine, -80 °C for storage.

Tea residue filtrate

A 250 mL three-necked flask was taken, 10 g of tea residue powder was weighed and added to 100 mL of water, which was placed in 37 °C water bath and stirred for 2 h. After cooling, the supernatant was filtered and taken for cell experiments.

Cell Lines and Plasmids

Human colon adenocarcinoma cells HT-29 cells and pEGFP-N1 plasmid were isolated and cultured and constructed by our own laboratory.

2.2. Animal Ethics Statement

According to the plan approved by the Animal Committee of Hunan Normal University, animal tests were carried out on the farm of Liuan Agricultural Science and Technology Comprehensive Development Co., LTD., Liuyang City, Hunan Province.

2.3. Animals and experimental design

This study was approved by the Animal Committee of Hunan Normal University. Thirty-six 21-day-old healthy weaned piglets (Duroc × Landrace × Yorkshire) with initial body weight were randomly divided into 2 treatments with 18 replicates per treatment and 1 pig per replicate. The control group was fed with the basal diet, and the experimental group added 1 kg/t of tea residue on the basis of the basic diet, and this dose was formulated according to the pre- experimental effect. The pre-feeding period was 3 days and the formal feeding period was 28 days. During the trial period, the piglets were fed 5 times a day, and the piglets were allowed to feed and drink freely and were kept in a single pen. The base diet was configured in accordance with the nutritional levels of weaned piglets in NRC (2012). The composition and nutrient content of the diets are shown in Table 1.

2.4. Sample collection and measurement

The abnormal behavior and diarrhea of the piglets were recorded daily throughout the test cycle. The piglets' feces were collected separately for 3 consecutive days on the 5th, 6th, 7th, 26th, 27th and 28th days of the experiment, and the collected feces were mixed and stored at -20 °C. On days 0, 7 and 28, the piglets were fasted (free to drink) after 10 pm, weighed and recorded the next morning, the average daily gain (ADG) was calculated. The amount of feed added and residuals per day were also recorded in detail to calculate the average daily feed intake (ADFI), and the feed utilization rate of piglets (G: F) was calculated according to the formula.

On days 1 and 21 of the experiment, 0.2% chromium trioxide was added to the diet to determine nutrient digestibility. On the 7th and 28th days of the experiment period, 6 piglets in each group were randomly selected for anterior vena cava blood collection, and the plasma was centrifuged and stored in the refrigerator at -80 °C. Feed samples of 1 kg were collected from each group separately and stored at -80 °C. At the end of the experiment, 6 piglets were randomly selected from each group. The piglets were euthanized by intravenous injection of 4% sodium pentobarbital solution. The intestine was isolated and the mesentery was removed, then rinsed with physiological saline, molecular samples of jejunum, ileum and colon were taken for 2 cm, wrapped in tin foil, frozen in liquid nitrogen and then stored in -80 °C refrigerator. About 2 cm of jejunum and ileum were taken separately and immersed in 10% formaldehyde solution, and the solution was changed after 24 h and stored at 4 °C.

2.5. Determination of nutrient digestibility

When the test was finished, the fecal samples were taken out and the 3 day fecal samples were mixed well. The samples were washed with 2% H2SO4 solution, baked in an oven (65 °C) for 72 h, and stored in a refrigerator at -80 °C. The crude protein, crude fiber, crude fat and total energy digestibility in the rations and manure samples were determined according to the methods in GB/T 6432-2018, GB/T 6433-2006, GB/T 6434-2006 and Feed Analysis and Feed Quality Testing Techniques, respectively. The chromium (Cr) content was determined by atomic absorption spectrometer (Tokyo, Japan) according to the national standard GB/T 13088-2006.

2.6. Plasma biochemical indicators

Plasma was removed from the refrigerator, thawed, centrifuged and analyzed for biochemical, antioxidant and immune index using Cobas C311 (Roche Diagnostics, Switzerland). Indicators included glutathione aminotransferase (AST), total protein (TP), alanine aminotransferase (ALT), alkaline phosphatase (ALP), albumin (ALB), cholesterol (TC), globulin (GLO), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), catalase (CAT), glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), superoxide dismutase (T-SOD) and immunoglobulins (IgG, IgA, IgM), total antioxidant capacity (T-AOC).The above kits were purchased from Weifang Sanwei Biological Engineering Co., LTD.

2.7. Morphological structure of the intestine

Samples of fixed jejunum and ileal intestinal tissue were removed, embedded in 1.5 cm sections of intestine, and cut into 4 mm thick sections. The sections were dried and stained with hematoxylin-eosin. Afterwards, intestinal morphology was observed and photographed with an orthochromatic fluorescence microscope (version 4.12, Leica Imaging Ltd., Cambridge, UK). Villi height (VH) and crypt depth (CD) were measured with Image Pro Plus 6.0 software, and the ratio of villi height to crypt depth (VH:CD) was calculated for each small intestine. At least 30 intact villous crypts were randomly selected and measured for each sample.

2.8. Determination of mineral fractions in feces

The fecal samples were taken out from the refrigerator, and the contents of mineral components such as calcium, phosphorus, sodium and chloride were detected. 0.20 g of the crushed samples were weighed and placed in a microwave digestion tank, 10 mL of concentrated nitric acid and 1 mL of hydrogen peroxide were added in turn, and the samples were digested using a microwave digestion apparatus. After boiling, add another 0.5mL of perchloric acid into the digestion tank and drive the acid away for 2h at 180 °C. Finally, the content of calcium, phosphorus, sodium and chlorine in the samples was determined by an inductively coupled plasma emission spectrometer (Agilent 5110 ICP-OES) with 1% nitric acid at a constant volume of 10mL. Its detection was conducted according to GB/T 19203-2003, GB/T 6437-2018, GB/T 40461-2021 and GB/T 24890-2010, respectively.

2.9. RNA extraction and cDNA synthesis

Total RNA was isolated from jejunal and colonic molecular sample tissues using TRIZOL reagent (TaKaRa, Beijing, China). mRNA reverse transcription kit (Prime Script TMRT Master Mix, Takala) instructions were followed: genomic DNA was removed first, followed by reverse transcription for cDNA synthesis.

2.10. Real-time fluorescence quantitative PCR

Primer design was performed using Primer premier 5.0, primer sequences were verified for specificity using Primer-BLAST, and finally primers were synthesized by Biotech. The primers and cDNA were diluted according to the recommended ratio in the reagent instructions. The reaction system (Quant-Studio, Thermo Fisher Scientific) was added, then transferred to a 384-well plate, mixed and reacted in a real-time fluorescence quantitative PCR instrument (ABI 7900HT, USA). The relative expression of mRNA in the target gene was corrected with β-actin. The primer sequences are shown in Table 2.

2.11. Target gene cloning and plasmid recombination

The enzymatic primers were designed using Snapgene software, and the PCR reactions were performed using the primers in Table 3 with cDNA as the template. And the product was purified using PCR purification kit (Beyoncé, D0033). The target gene TMEM16A and pEGFP-N1 vector were cut by BmtⅠ/BamHⅠenzyme, and the target gene CFTR and pEGFP-N1 vector were cut by XhoⅠ/AgeⅠenzyme. Gel Extraction Kit D2500(Omega) was used to recover enzymic PCR products and pEGFP-N1 vector, which were then linked with T4 DNA ligase to transform Escherichia coli receptor cells DH5α (Thermo Fisher, EC0112), and select cloned plasmids. The correct plasmid was selected for DNA sequence analysis after enzyme digestion identification.

2.12. Transformation, extraction and purification of recombinant plasmids

The receptor cells were mixed with the recombinant plasmid, and the culture was expanded by thermal shock, then the culture was applied on LB agar (containing neomycin) plates and incubated in a constant temperature chamber at 37 °C for 12-16 h. The monoclonal clones were inoculated in LB liquid medium containing neomycin resistance. 2mL of the DH5α bacterial solution identified as positive was sent to Shanghai Meiji Biomedical Technology Co.

2.13. Transient transfection of cells

HT-29 cells were cultured until the cell density was about 70% to 90%, washed twice with PBS, 1 mL of serum-free medium was added, and then the diluted plasmid to be transfected and transfection reagent were added and mixed, and the culture was continued with normal culture medium after 4-6 h. The cells expressing EGFP were observed by inverted fluorescence microscopy for preliminary detection of plasmid expression. Electrophysiological tests can be performed 24-48 h after transfection.

2.14. Electrophysiological recordings

Whole cell patch-clamp experiment was recorded at room temperature. The pEGFP-N1-CFTR and pEGFP-N1-TMEM16A HT-29 cells were taken separately, cultured on small slides, and after the cells were plastered, the cell smears were gently rinsed with Tait's solution 2-3 times and placed in a perfusion bath. Adjust the perfusion speed; install Ag/AgCl electrode so that the electrode resistance in the bath is 3-5 MΩ; adjust the inverted phase contrast microscope (Olympus, Japan), move the micromanipulator close to the cells to be examined, and reduce the positive pressure inside the electrode appropriately to seal the cells; compensate the electrode capacitance, and then increase the negative pressure inside the microelectrode to break the cell membrane; form a whole-cell recording mode. The membrane potential is held at 0 mV using a patch-clamp amplifier, and the membrane potential is measured between -100 mV and +100 mV by pulsing the software in order to create a current-voltage (I-V) relationship. Cellular current assays were performed by compensating for whole-cell membrane capacitance and series resistance, correcting for leakage current by adding the chloride channel activators trichothecene and ionomycin to the cell bath and comparing the current to that of a typical chloride ion to detect its current characteristics. Tea residue filtrate was added to the cell bath solution and whole cell recordings were made at room temperature using a membrane clamp amplifier on cells that emitted EGFP fluorescence.

2.15. Statistical analysis

Data are expressed as means and SEM is the total standard error of the total sample mean. Data were analyzed using SPSS 25.0 software (IBM Corp., Chicago, IL, USA) to test the data according to normal distribution, and then analyzed using independent samples t-test to determine statistical differences between groups according to Duncan's method, where 0.05 ≤ P < 0.10 is a trend of difference, P< 0.05 is a difference significant, and P < 0.01 was a highly significant difference.

3. Results

3.1. Effect of growth performance

Table 4 shows the effect of feed supplementation with tea residue on growth performance. Supplementation of the diet with 1 kg/t of tea reside had no significant effect on body weight (BW), average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (G: F) of weaned piglets (P > 0.05).

3.2. Diarrhea rate

Compared to the basal diet group, the addition of 1 kg/t tea residue to the diet significantly reduced the diarrhea rate in weaned piglets at d 1-7 and d 1-28 (P < 0.01), however, there was no significant effect on the diarrhea rate at d 8-28 (Table 5).

3.3. Nutrient digestibility

The effect of tea residue on nutrient digestibility of weaned piglets is shown in Table 6. There were no significant differences in crude protein, crude fat, crude fiber and total energy digestibility between the two groups (P >0.05).

3.4. Plasma biochemical indexes

As shown in Table 7, the addition of tea residue to the diet had no significant effect on the plasma biochemical indices of total protein, albumin, globulin, cholesterol, triglycerides, AST, ALT, ALP, HDL and LDL in weaned piglets (P > 0.05).

3.5. Effect on plasma antioxidant indexes

As shown in Table 8, compared with the basal diet group, there was no significant effect (P > 0.05) on antioxidant indices such as CAT, GSH-Px, T-SOD, T-AOC and MDA in weaned piglets fed the tea residue diet, but it significantly reduced the level of glutathione (GSH) at d 28 (P < 0.05).

3.6. Effect on plasma immune indexes

As shown in Table 9, the addition of tea residue to the diet had no significant effect (P > 0.05) on IgA, IgG and IgM plasma immune indexes in weaned piglets.

3.7. Effect on the morphological structure of the intestine

As shown in Table 10, the addition of 1 kg/t of tea residue to the diet fed to piglets had no significant effect on jejunum, ileum villus height, crypt depth and VH/CD (P > 0.05).

3.8. Effect on fecal mineral fraction

As shown in Table 11, the addition of tea residue to the diet reduced the fecal chloride content of weaned piglets very significantly (P < 0.01) compared to the basal diet group, but there was no significant difference in the content of mineral fractions such as calcium, phosphorus and sodium in the feces (P > 0.05).

3.9. Effect on mRNA expression of colonic chloride channels

As shown in Figure 1, the addition of tea residue to the diet had no significant effect on the mRNA expression of the colonic chloride channels TMEM16A and CFTR in weaned piglets.

3.10. Effect on electrophysiological activity of colonic chloride channels

Figure 2 shows the current-voltage (I-V) curves obtained for TMEM16A and CFTR under different treatment conditions with a cell clamp potential of 0 mV and a test potential obtained by gradually changing from -100 mV to +100 mV (step voltage of 20 mV). Whole-cell membrane clamp recordings showed that TMEM16A ion channel current was positively correlated with the change in voltage, with a small increase at negative charges and an increase in current change with increasing positive voltage. Ionomycin activated TMEM16A to produce chloride current and this current was significantly reduced after tea residue filtrate treatment, indicating that TMEM16A could be significantly inhibited by tea residue. Whole-cell membrane clamp recordings of CFTR ion channels showed that the current was positively correlated with the change in voltage. Forskolin activated CFTR channels to generate chloride current and this current was significantly reduced after tea residue filtrate treatment, indicating that CFTR can be significantly inhibited by tea residue.

4. Discussion

Weaning is an important stage of piglet growth. The gastrointestinal tract is the largest immune organ of the organism and protects the body from various pathogens, antigens, endotoxins and potentially harmful microorganisms [22]. The main target of weaning stress is in the intestine, which causes some damage to the morphological structure of the piglet intestine and affects the intestinal function [23], manifested by oxidative damage, barrier destruction and reduced digestion and absorption capacity [24]. This results in reduced feed intake, slower growth and increased morbidity in piglets. Under stressful conditions, piglets are more likely to be infected with pathogenic bacteria that further damage intestinal structure and function, resulting in or aggravating vomiting and diarrhea, which can endanger piglet health, increase piglet mortality, and cause huge economic losses [3,25]. Feeding antibiotics can effectively relieve post-weaning diarrhea and improve piglet growth performance, but the long-term overuse of antibiotics leads to bacterial resistance and antibiotic residues in animal products, threatening human health [26]. Recently, it has been shown that algal polysaccharides can be used to reduce oxidative stress and inflammation to protect intestinal function [27]. Some studies have shown fermented tea, which has various functional properties such as anti-inflammatory potential and antioxidant activity [28], may contribute to the improvement of intestinal function.

Tea is an important cash crop in China with the advantages of being nontoxic and green. It is reported that about 90% of tea leaves become tea residue after processing and consumption [29]. The polyphenolic substances in tea have functions such as antioxidant and antibacterial. However, polyphenolic substances can also affect the taste of tea, and when tea polyphenols are present in excess, they can exhibit a bitter taste, and when tea residue are added to the ration, it can affect its palatability. Feeding diets with added tea residue can have different degrees of effect on different kinds of animals. Studies have shown that fermented herbal tea residue can effectively reduce the respiratory rate and rectal temperature of fattening cattle under heat stress and increase daily feed intake and daily weight gain [30]. When black or green tea and its tea residue were added to sheep diets, their rumen function was optimized and methane emissions were reduced [31]. As explored by this experiment, there was no significant difference in the growth performance of piglets fed diets supplemented with tea residue compared to the control group, which is consistent with Zhaoming Yan [32], indicating that tea residue has no adverse effect on the growth performance of weaned piglets.

Digestibility is an important index to measure the digestibility of animals and feed digestibility. When piglets are weaned, because their digestive system is not yet mature, piglets often have problems such as insufficient secretion of gastric acid and low activity of digestive enzymes in the intestine, which cause a decrease in the digestion and absorption of nutrients in the diet of weaned piglets and affect their growth performance [33]. Mohammed Rashed Chowdhury showed that feeding a diet supplemented with heat-treated green tea increased the apparent crude protein digestibility of Black Bengal goats [34]. Studies showed that the addition of fermented tea residue (equal percentage replacement of concentrate supplement) had no significant effect on digestibility of organic matter, crude protein and dry matter in lake goats [35]. Which was similar to the results of the present study, and tea residue did not cause significant differences in crude protein, crude fat, crude fiber and total energy digestibility in weaned piglets.

Plasma biochemical indicators reflect the health status of the organism. The addition of tea residue to the diet had no significant effect on the plasma biochemical indices of total protein, albumin, globulin, cholesterol, triglycerides, AST, ALT, ALP, HDL and LDL in weaned piglets. This is basically consistent with the results of Wang Guiyun's study, thus indicating that tea residue has no negative effect on the plasma biochemical functions of weaned piglets.

The antioxidant defense system in the body has a key role in the normal growth of animals and the removal of harmful substances such as oxygen free radicals from the body. Tea polyphenols can improve antioxidant capacity and glutathione peroxidase activity and reduce malondialdehyde content in growing fattening pigs, and their antioxidant capacity is influenced by the spatial configuration, which is generally positively correlated with the number of hydroxyl groups [36,37]. When fermented tea residue was added to the diets of fattening pigs, it significantly increased glutathione peroxidase activity and total antioxidant capacity [38]. In the present study, the addition of tea residue had no significant effect on the activities of various antioxidant enzymes in weaned piglets compared with the control group, but it increased the plasma glutathione content at day 28, indicating that tea residue has positive effects in antioxidant and maintaining normal physiological functions of the body.

The effect of tea and tea residue is mainly dependent on the active ingredients it contains, such as tea polysaccharides, tea polyphenols, L-theanine, tea pigments and other common components. When Fuzhuan tea polysaccharide was added to the diet of cyclophosphamide-treated mice, it restored the levels of cytokines and immunoglobulins and effectively regulated the immune function of mice [39]. In the present study, however, there was no significant effect on plasma immune indexes in weaned piglets.

The intestinal barrier is an important way for the intestinal epithelium to effectively prevent the invasion of toxic and harmful substances [40]. A normal intestinal structure is an important foundation for healthy and fast growth of piglets, and weaning stress in piglets can damage the intestinal structure with problems such as intestinal villi atrophy and increased crypt depth, which can have an impact on digestion, absorption, secretion and barrier function [41]. Yixuan Bai found that Fuzhuan tea polysaccharides improved cyclophosphamide-induced intestinal damage in mice and effectively restored their intestinal morphology [39]. Green tea polyphenols dose-dependently improved intestinal epithelial homeostasis in mice and showed superior anti-inflammatory effects and colonic barrier integrity [42]. In the present study, no differential changes in the intestinal morphology of piglets fed tea residue was observed compared to the control group. Thus, it appears that tea residue has no adverse effect on the morphological structure of the intestinal mucosa of piglets.

Much evidence suggests that herbs such as tea can be effective against diarrhea [19]. Considering the special method of making Fuzhuan tea, it is also possible that it is the result of the action of fermenting microorganisms and their metabolites and active substances. There are studies to reduce diarrhea in the first two weeks after weaning by developing feeding strategies [43]. In the present study, by recording diarrhea in piglets, it was found that the addition of tea residue to the diet significantly reduced the diarrhea rate from 1-7 d and 1-28 d. This shows that tea residue have a good anti-diarrheal effect and have a wide scope for feed development and the development of anti-diarrheal products. In this study, the addition of tea residue to the diet also significantly reduced the fecal chloride content in weaned piglets. From this, we can tentatively determine that tea residue can promote the absorption of chloride ions, and since watery diarrhea is mainly due to excessive excretion of sodium and chloride ions from the intestine, it is tentatively inferred that it is caused by abnormal expression or dysfunction of chloride channels in the colon.

TMEM16A is a calcium-dependent chloride channel whose activity is controlled by cytoplasmic calcium concentration. It plays an important physiological role in many organ systems. Overexpression of TMEM16A will lead to pulmonary hypertension [44], hypertension [45], cancer [46] and so on. TMEM16A mediated CaCC is regulated by calmodulin-dependent protein kinase II (CaMKII) and protein phosphatase 1/protein phosphatase 2A (PP1/PP2A) [47]. CFTR can be regulated by AMP protein-activated kinase (AMPK) [48], it is the main channel for chloride secretion in the intestine. It was found that the addition of tea residue to the diet significantly reduced the fecal chlorine content in weaned piglets. From this, we can tentatively determine that tea residue can promote the absorption of chloride ions, and since watery diarrhea is mainly due to excessive excretion of sodium and chloride ions from the intestine, it is tentatively inferred that it is due to abnormal expression or dysfunction of chloride channels in the colon. However, there was no significant effect on the relative expression of mRNA of colonic ion channels TMEM16A and CFTR in weaned piglets, and combined with cell culture and electrophysiological experiments, it was further verified that tea residue extract could significantly inhibit the opening of TMEM16A and CFTR ion channels in HT-29 cells, thus improving the diarrhea in weaned piglets.

5. Conclusion

In conclusion, the addition of tea residue to the diet had no effect on the growth performance and digestibility of piglets, but to some extent, it could enhance the antioxidant function of piglets and significantly reduce the rate of post-weaning diarrhea. Dietary tea residue can significantly reduce the content of chloride ions in feces, but has no significant effect on the relative mRNA expression of the colon ion channels TMEM16A and CFTR in weaned piglets. Combined with cell culture and electrophysiological experiments, it was further verified that tea residue extract could significantly inhibit the opening of TMEM16A and CFTR ion channels in HT-29 cells, thus improving diarrhea in weaned piglets.