Wang, H.; Hou, J.; Wang, D.; Yang, M.; Liu, J. Enhancement of Lycopene Synthesis via Low-Frequency Alternating Magnetic Field in Brassica trispora. Fermentation2024, 10, 69.
Wang, H.; Hou, J.; Wang, D.; Yang, M.; Liu, J. Enhancement of Lycopene Synthesis via Low-Frequency Alternating Magnetic Field in Brassica trispora. Fermentation 2024, 10, 69.
Wang, H.; Hou, J.; Wang, D.; Yang, M.; Liu, J. Enhancement of Lycopene Synthesis via Low-Frequency Alternating Magnetic Field in Brassica trispora. Fermentation2024, 10, 69.
Wang, H.; Hou, J.; Wang, D.; Yang, M.; Liu, J. Enhancement of Lycopene Synthesis via Low-Frequency Alternating Magnetic Field in Brassica trispora. Fermentation 2024, 10, 69.
Abstract
In recent years, magnetic fields have emerged as a non-thermophysical treatment with a signifi-cant impact on microbial fermentation processes. Brassica trispora is a microorganism known for its indus-trial-scale production of lycopene and high yield of single cells. This study aimed to in-vestigate the impact of low frequency magnetic fields on lycopene synthesis by Brassica trispora and elucidate the underlying mechanism for enhancing lycopene yield. The results indicated that both the intensity and duration of the magnetic field treatment influenced the cell. Exposing the cell to a 0.5 mT magnetic field for 48 hours on the second day of fermentation resulted in a lyco-pene yield of 1473.16 mg/L, representing a re-markable increase of 216.1% compared to the con-trol group. Transcriptome analysis revealed that al-ternating magnetic field significantly up-regulated genes related to ROS and cell membrane structure, leading to a substantial increase in lycopene production. Scanning electron microscopy revealed that the magnetic field treatment resulted in rough, loose, wrinkled surface morphology of the mycelium, along with a few mi-cropores, thereby altering the cell membrane permeability to some extent. Moreover, there was a significant increase in intracellular ROS content, cell membrane permeability, key enzyme activi-ty involved in lycopene metabolism, and ROS-related enzyme activity. In conclusion, the alter-nating fre-quency magnetic field can activate a self-protective mechanism that enhances lyco-pene synthesis by modulating intracellular ROS content and cell membrane structure. These find-ings not only deepen our understanding of the impact of magnetic fields on microbial growth and metabolism but also provide valuable insights for developing innovative approaches to en-hance carotenoid fermentation.
Keywords
magnetic field; lycopene; Brassica trispora; transcriptome; reactive oxygen species
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
