Version 1
: Received: 28 November 2023 / Approved: 30 November 2023 / Online: 30 November 2023 (10:49:46 CET)
How to cite:
Kim, M.; Kim, J.; Lee, S.; Kim, H.; Taylor, R.A.; Kyhm, K. Photobleaching and Polarization Anisotropy of Olive Flounder Canine by Nonlinear Optical Microscopy. Preprints2023, 2023111931. https://doi.org/10.20944/preprints202311.1931.v1
Kim, M.; Kim, J.; Lee, S.; Kim, H.; Taylor, R.A.; Kyhm, K. Photobleaching and Polarization Anisotropy of Olive Flounder Canine by Nonlinear Optical Microscopy. Preprints 2023, 2023111931. https://doi.org/10.20944/preprints202311.1931.v1
Kim, M.; Kim, J.; Lee, S.; Kim, H.; Taylor, R.A.; Kyhm, K. Photobleaching and Polarization Anisotropy of Olive Flounder Canine by Nonlinear Optical Microscopy. Preprints2023, 2023111931. https://doi.org/10.20944/preprints202311.1931.v1
APA Style
Kim, M., Kim, J., Lee, S., Kim, H., Taylor, R.A., & Kyhm, K. (2023). Photobleaching and Polarization Anisotropy of Olive Flounder Canine by Nonlinear Optical Microscopy. Preprints. https://doi.org/10.20944/preprints202311.1931.v1
Chicago/Turabian Style
Kim, M., Robert A. Taylor and Kwangseuk Kyhm. 2023 "Photobleaching and Polarization Anisotropy of Olive Flounder Canine by Nonlinear Optical Microscopy" Preprints. https://doi.org/10.20944/preprints202311.1931.v1
Abstract
We have investigated the canine of olive flounder in terms of the nonlinear optical signals of two-photon excitation autofluorescence (2PEF) and second harmonic generation (SHG), whereby two kinds of nonlinear microscope images of the canine were obtained using ~140 femtosecond pulses. For increasing excitation power of laser pulses, a photobleaching effect was found to be significant in the red and green ranges of autofluorescence, where a double-exponential decay of the intensity occurs within hundreds of second due to collagen fluorophores in the canine. In the blue range of autofluorescence, the photobleaching is suppressed due to an energy conversion of the incident laser pulse to SHG signal. As a result, the signal in blue range remains constant for long term exposure to laser. We have also found that the SHG intensity depends on the orienta-tion of linearly polarized excitation, and the optical anisotropy of SHG can be attributed to the collagen alignment of canines.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
