Streeter, A.R.; Cartwright, A.; Zargaran, M.; Wankhade, A.; Anderson, A.J.; Britt, D.W. Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6. Agrochemicals2024, 3, 1-11.
Streeter, A.R.; Cartwright, A.; Zargaran, M.; Wankhade, A.; Anderson, A.J.; Britt, D.W. Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6. Agrochemicals 2024, 3, 1-11.
Streeter, A.R.; Cartwright, A.; Zargaran, M.; Wankhade, A.; Anderson, A.J.; Britt, D.W. Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6. Agrochemicals2024, 3, 1-11.
Streeter, A.R.; Cartwright, A.; Zargaran, M.; Wankhade, A.; Anderson, A.J.; Britt, D.W. Adjuvant Pluronic F68 Is Compatible with a Plant Root-Colonizing Probiotic, Pseudomonas chlororaphis O6. Agrochemicals 2024, 3, 1-11.
Abstract
Plant probiotic bacteria are being increasingly used to maximize productivity and quality of field crops. Pseudomonas chlororaphis O6 (PcO6) is a plant root colonizer with probiotic activities that produces metabolites such as phenazines functional in plant protection. This work reports responses of PcO6 to a non-ionic, triblock copolymer surfactant, Pluronic F68. This Pluronic exhibits membrane “healing” activity and improves cryopreservation recovery in eukaryotic cells. The product is FDA approved and is applied as an adjuvant in formulations used in agriculture, medicine, and biotechnology. Growth of PcO6 on Luria broth at 25°C was unhindered by 0.1 and 1.0 g/L F68, reduced at 10 g/L, with more inhibition at 100 g/L F68; micelle formation could account for inhibited growth at higher doses. Phenazine production was not changed by F68, whereas an F68 dose-dependent, surfactant-induced spread of bacterial colonization on 0.5% agar was observed. Exposure of cells to fluorescently labelled F68 resulted in intense fluorescence, stable to washing, showing a direct association of the Pluronic with the bacterium. However, neither protection nor harm was found for PcO6 cells undergoing repetitive freeze (-20 °C)/thaw cycles with 0.1% or 1% F68. These findings suggest F68 could be compatible for use in agricultural formulations with little effect on probiotics such as PcO6.
Environmental and Earth Sciences, Environmental Science
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