Version 1
: Received: 20 November 2023 / Approved: 21 November 2023 / Online: 21 November 2023 (07:43:24 CET)
Version 2
: Received: 15 February 2024 / Approved: 16 February 2024 / Online: 16 February 2024 (07:00:07 CET)
How to cite:
CANATAR, İ.; ÖZDAŞ, S.; Baydemir Pesint, G. Phyllanthus emblica Loaded Cryogels for Improved Wound Care: Characterizationand In Vitro Studies. Preprints2023, 2023111288. https://doi.org/10.20944/preprints202311.1288.v2
CANATAR, İ.; ÖZDAŞ, S.; Baydemir Pesint, G. Phyllanthus emblica Loaded Cryogels for Improved Wound Care: Characterizationand In Vitro Studies. Preprints 2023, 2023111288. https://doi.org/10.20944/preprints202311.1288.v2
CANATAR, İ.; ÖZDAŞ, S.; Baydemir Pesint, G. Phyllanthus emblica Loaded Cryogels for Improved Wound Care: Characterizationand In Vitro Studies. Preprints2023, 2023111288. https://doi.org/10.20944/preprints202311.1288.v2
APA Style
CANATAR, İ., ÖZDAŞ, S., & Baydemir Pesint, G. (2024). <strong><em></em></strong><em>Phyllanthus emblica</em> Loaded Cryogels for Improved Wound Care: Characterizationand In Vitro Studies. Preprints. https://doi.org/10.20944/preprints202311.1288.v2
Chicago/Turabian Style
CANATAR, İ., Sibel ÖZDAŞ and Gözde Baydemir Pesint. 2024 "<strong><em></em></strong><em>Phyllanthus emblica</em> Loaded Cryogels for Improved Wound Care: Characterizationand In Vitro Studies" Preprints. https://doi.org/10.20944/preprints202311.1288.v2
Abstract
Phyllanthus emblica (P. emblica) has been described in many studies to have multiple therapeutic activities and is considered an important part of Ayurvedic and Unani medicinal systems. This study successfully synthesized P. emblica loaded polyvinylalcohol/gelatin based cryogels (PVA/Gel/P.emblica) to examine their potential in wound dressing applications. Swelling tests, Brunauer-Emmett-Teller (BET) and morphology analysis were conducted within the scope of characterization studies. In vitro cell culture studies were performed using HaCaT cells to illustrate the suitability of the synthesized cryogels as wound dressing material. The swelling tests and BET analysis results showed that swelling and surface area properties of cryogels increased with increasing P. emblica amounts. Morphological results displayed that the cryogels had a dense, interconnected pore morphology and a macroporous structure. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue exclusion and live-dead assay results revealed that adding of P. emblica into cryogels led to enhanced cell proliferation, increased cell number, and improved cell viability. Based on the phase contrast microscope, scanning electron microscope (SEM), immunofluorescence and giemsa staining images, it was observed that P. emblica promoted cell attachment, proliferation and penetration. In conclusion, it has been shown that PVA/Gel/P.emblica cryogels are suitable for use as wound dressing materials and can be developed with further studies.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
