Version 1
: Received: 20 November 2023 / Approved: 20 November 2023 / Online: 20 November 2023 (09:29:03 CET)
How to cite:
Iyer, S.; Hattori, N.; Okuda, H.; Nakagawa, T.; Maeda, T.; Koseki, H.; Ito, T. Dissecting the Impact of USP21 on Mouse Lipid Metabolism and Identifying Polymorphism-Associated Hypercholesterolemia in Outpatients. Preprints2023, 2023111236. https://doi.org/10.20944/preprints202311.1236.v1
Iyer, S.; Hattori, N.; Okuda, H.; Nakagawa, T.; Maeda, T.; Koseki, H.; Ito, T. Dissecting the Impact of USP21 on Mouse Lipid Metabolism and Identifying Polymorphism-Associated Hypercholesterolemia in Outpatients. Preprints 2023, 2023111236. https://doi.org/10.20944/preprints202311.1236.v1
Iyer, S.; Hattori, N.; Okuda, H.; Nakagawa, T.; Maeda, T.; Koseki, H.; Ito, T. Dissecting the Impact of USP21 on Mouse Lipid Metabolism and Identifying Polymorphism-Associated Hypercholesterolemia in Outpatients. Preprints2023, 2023111236. https://doi.org/10.20944/preprints202311.1236.v1
APA Style
Iyer, S., Hattori, N., Okuda, H., Nakagawa, T., Maeda, T., Koseki, H., & Ito, T. (2023). Dissecting the Impact of USP21 on Mouse Lipid Metabolism and Identifying Polymorphism-Associated Hypercholesterolemia in Outpatients. Preprints. https://doi.org/10.20944/preprints202311.1236.v1
Chicago/Turabian Style
Iyer, S., Haruhiko Koseki and Takashi Ito. 2023 "Dissecting the Impact of USP21 on Mouse Lipid Metabolism and Identifying Polymorphism-Associated Hypercholesterolemia in Outpatients" Preprints. https://doi.org/10.20944/preprints202311.1236.v1
Abstract
USP21, a member of the ubiquitin protease family, plays a vital role in various biological functions. However, the effects of USP21 dysfunction have yet to be fully understood. Here, we generated Usp21 knockout (KO) mice and conducted microarray to identify genes that Usp21 regulates. Its results raised several genes based on their biological characteristics, and RT-qPCR confirmed their expression. KO mice exhibited significant elevations in the expression of the genes Fabp7 and Nlrc5, which play a role in lipid metabolisms, compared to wild-type mice. Blood tests showed no impairment of liver function but did reveal elevated levels of total cholesterol and free fatty acids in KO mice compared to wild-type mice. These data suggest that Usp21 may play roles in lipid metabolism associated with Fabp7 and Nlrc5. To clarify the involvement of USP21 in human hypercholesterolemia, we examined single nucleotide polymorphisms (SNPs) around USP21 in non-hypercholesterolemic and hypercholesterolemic outpatients. We found that the rs11421 SNP downstream of USP21 was associated with hypercholesterolemia. These data suggest that USP21 plays a role in mice and human lipid metabolism and that its polymorphism may be a diagnostic marker for human hypercholesterolemia.
Keywords
USP21; Fabp7; Nlrc5; hypercholesterolemia
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
