Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Radiolabeling of Platelets with 99mTc-HYNICDuramycin for In Vivo Imaging Studies

Keresztély Merkel
,
Dávid Szöllősi
,
Ildiko Horvath
,
Jezsó Bálint
ORCID logo ,
Zsolt Baranyai
,
Krisztián Szigeti
,
Zoltán Varga
* ,
Imre Hegedüs
,
Parasuraman Padmanabhan
ORCID logo ,
Balázs Gulyás
,
Ralf Bergmann
ORCID logo ,
Domokos Máthé
*
Version 1 : Received: 2 November 2023 / Approved: 7 November 2023 / Online: 7 November 2023 (15:09:47 CET)

A peer-reviewed article of this Preprint also exists.

Merkel, K.; Szöllősi, D.; Horváth, I.; Jezsó, B.; Baranyai, Z.; Szigeti, K.; Varga, Z.; Hegedüs, I.; Padmanabhan, P.; Gulyás, B.; Bergmann, R.; Máthé, D. Radiolabeling of Platelets with 99mTc-HYNIC-Duramycin for In Vivo Imaging Studies. Int. J. Mol. Sci. 2023, 24, 17119. Merkel, K.; Szöllősi, D.; Horváth, I.; Jezsó, B.; Baranyai, Z.; Szigeti, K.; Varga, Z.; Hegedüs, I.; Padmanabhan, P.; Gulyás, B.; Bergmann, R.; Máthé, D. Radiolabeling of Platelets with 99mTc-HYNIC-Duramycin for In Vivo Imaging Studies. Int. J. Mol. Sci. 2023, 24, 17119.

Abstract

(1) Background: Following the in vivo biodistribution of platelets can contribute to a better un-derstanding of their physiological and pathological roles, and nuclear imaging methods, such as single photon emission tomography (SPECT), provide an excellent method for that. SPECT imaging needs stable labeling of the platelets with a radioisotope. (2) Methods: In this study, we report a new method to label platelets with 99mTc, the most frequently used isotope for SPECT in clinical applications. The proposed radiolabeling procedure uses a membrane-binding peptide, duramycin. (3) Results: Our results show that duramycin does not cause significant platelet activation and radiolabeling can be carried out with a procedure utilizing a simple labeling step followed by a size-exclusion chromatography-based purification step. 4) Conclusions: The performed small animal SPECT/CT in vivo imaging investigations revealed good in vivo stability of the labeling, which paves the way for further application of 99mTc-HYNIC-Duramycin in platelet imaging.

Keywords

platelet radiolabeling; 99mTc-HYNIC-Duramycin; SPECT

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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