Combination of Blue Light 460nm and H2O2 Is Safe and Effective to Eradicate Staphylococcus aureus in an Infected Mouse Skin Abrasion Model

Vu Nguyen Ngo; Thien Nguyen Thuan Truong; Tin Trung Tran; Loan Thanh Nguyen; Ngoc Bao Mach; Van Van Vu; Thi Thu Hoai Nguyen; Thiet Minh Vu

doi:10.20944/preprints202311.0021.v1

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preprints.org > biology and life sciences > biochemistry and molecular biology > doi: 10.20944/preprints202311.0021.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Combination of Blue Light 460nm and H2O2 Is Safe and Effective to Eradicate Staphylococcus aureus in an Infected Mouse Skin Abrasion Model

Vu Nguyen Ngo

Thien Nguyen Thuan Truong

Version 1 : Received: 31 October 2023 / Approved: 1 November 2023 / Online: 1 November 2023 (09:26:39 CET)

A peer-reviewed article of this Preprint also exists.

Ngo, V.N.; Truong, T.N.T.; Tran, T.T.; Nguyen, L.T.; Mach, N.B.; Vu, V.V.; Nguyen, T.T.H.; Vu, T.M. A Combination of Blue Light at 460 nm and H₂O₂ for the Safe and Effective Eradication of Staphylococcus aureus in an Infected Mouse Skin Abrasion Model. Microorganisms 2023, 11, 2946. Ngo, V.N.; Truong, T.N.T.; Tran, T.T.; Nguyen, L.T.; Mach, N.B.; Vu, V.V.; Nguyen, T.T.H.; Vu, T.M. A Combination of Blue Light at 460 nm and H2O2 for the Safe and Effective Eradication of Staphylococcus aureus in an Infected Mouse Skin Abrasion Model. Microorganisms 2023, 11, 2946.

Abstract

Antibiotic-free approaches are more important than ever to deal with the rapidly growing problem of antibiotic resistance. The blue light 460nm (BL460nm) has been shown to effectively attenuate Staphylococcus aureus to chemical and physical agents through photolysis of the bacterial virulence factor Staphyloxanthin. However, the phototherapy using BL640nm has yet to be investigated in detail for its safety in eradicating Staphylococcus aureus in vitro and in vivo. In this study, we used a LED source producing a continuous wavelength of 460 nm along with hydrogen peroxide to treat S. aureus in culturing conditions and a wound abrasion mouse model. The results proved the safety of the combined therapy when it did not modify the bacterial virulence factors or the susceptibility to the widely used antibiotics. In addition, the experimental results on the mouse skin infection model also showed that the combined therapy is safe to apply to mouse skin and that it did not cause adverse skin irritation. Furthermore, the therapy can help Staphylococcus aureus-infected wounds heal with efficacy comparable to the topical antibiotic Fucidin. The above results suggest that the combination therapy of 460nm blue light and hydrogen peroxide can be safely used as an alternative to or adjunct to antibiotics in treating S. aureus-infected wounds.

Keywords

Blue light 460nm; hydrogen peroxide; safety; skin infection; Staphylococcus aureus; Staphyloxanthin

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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