Nikolic, G.; Zivotic, M.; Mioljevic, A.; Despotovic, S.; Nikolic, S.N.; Rabasovic, M.; Radojevic Skodric, S.; Matija, L. New Insights into Renal Neoplasm Differentiation: Nonlinear Microscopy's Breakthrough Advantages. Preprints2023, 2023101864. https://doi.org/10.20944/preprints202310.1864.v1
APA Style
Nikolic, G., Zivotic, M., Mioljevic, A., Despotovic, S., Nikolic, S.N., Rabasovic, M., Radojevic Skodric, S., & Matija, L. (2023). New Insights into Renal Neoplasm Differentiation: Nonlinear Microscopy's Breakthrough Advantages. Preprints. https://doi.org/10.20944/preprints202310.1864.v1
Chicago/Turabian Style
Nikolic, G., Sanja Radojevic Skodric and Lidija Matija. 2023 "New Insights into Renal Neoplasm Differentiation: Nonlinear Microscopy's Breakthrough Advantages" Preprints. https://doi.org/10.20944/preprints202310.1864.v1
Abstract
Multiphoton microscopy (MPM) is a cutting-edge nonlinear microscopy technique known for its exceptional capabilities in imaging dense tissues, making it an ideal tool for exploring human kidney tissue. This study delves into the application of MPM in evaluating renal cell tumors and characterizing tumor capsules. MPM encompasses two core methods: two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG). It leverages femtosecond lasers to provide deep tissue imaging while minimizing phototoxicity, making it well-suited for live tissue analysis. The study analyzed 100 formalin-fixed paraffin-embedded (FFPE) kidney tissue samples, ensuring compliance with ethical standards. Using a Ti:Sapphire laser for femtosecond pulses and a variety of optical components and filters, the researchers captured TPEF and SHG signals, facilitating image analysis. Normal kidney tissue exhibited distinctive structures like glomeruli and tubules under MPM. Renal cell carcinoma (ccRCC) displayed unique TPEF patterns with eccentrically located nuclei and indistinct cytoplasmic membranes. Papillary RCC (pRCC) exhibited a papillary pattern with dark nuclei, while chromophobe RCC (chRCC) showcased distinct features like thick cell membranes, strong TPEF signal in nuclei, and granular cytoplasm. Renal oncocytoma (RO) presented strong TPEF signals in cuboidal cells with microcyst-like arrangements. Overall, MPM proved invaluable in assessing human kidney tissue, offering distinct TPEF patterns for different renal cell tumor types. This non-invasive technique has the potential to enhance pathological examinations and advance our understanding of complex biological processes, ultimately contributing to improved diagnostics and treatment strategies for renal diseases.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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