Vieira, A.F.C.; Xatse, M.A.; Murray, S.Y.; Olsen, C.P. Oleic Acid Metabolism in Response to Glucose in C. elegans. Metabolites2023, 13, 1185.
Vieira, A.F.C.; Xatse, M.A.; Murray, S.Y.; Olsen, C.P. Oleic Acid Metabolism in Response to Glucose in C. elegans. Metabolites 2023, 13, 1185.
Vieira, A.F.C.; Xatse, M.A.; Murray, S.Y.; Olsen, C.P. Oleic Acid Metabolism in Response to Glucose in C. elegans. Metabolites2023, 13, 1185.
Vieira, A.F.C.; Xatse, M.A.; Murray, S.Y.; Olsen, C.P. Oleic Acid Metabolism in Response to Glucose in C. elegans. Metabolites 2023, 13, 1185.
Abstract
A key response to glucose stress is an increased production of unsaturated fatty acids to balance the increase of saturated fatty acids in the membrane. The C. elegans homolog of stearoyl-CoA desaturase, FAT-7, introduces the first double bond into saturated C18 fatty acids yielding oleic acid and is a critical regulatory point for surviving cold and glucose stress. Here, we incorporated 13C stable isotopes into the diet of nematodes and quantified the 13C-labelled fatty acid by GC-MS and HPLC/MS-MS to track the metabolic response to various concentrations of glucose. Previous work has analyzed membrane composition of C. elegans when responding to mild glucose stress and showed few alterations in overall fatty acid composition in the membrane. Here, in nematodes exposed to higher concentrations of glucose, a specific reduction of oleic acid and linoleic acid was observed. Using time courses and stable isotope tracing, the response of fatty acid me-tabolism to increasing levels of glucose stress has been characterized, revealing a funneling of monounsaturated fatty acids to preserve polyunsaturated fatty acid abundance. Taken together, higher levels of glucose unveil a specific reduction of oleic and linolenic acid in the metabolic re-wiring required to survive glucose stress.
Biology and Life Sciences, Biochemistry and Molecular Biology
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