Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies

Franklin Loic Tchinda Taghu
ORCID logo ,
Boniface Pone Kamdem
ORCID logo ,
Vincent Ngouana
,
Zuriatou Yajep Tanka
,
Victorine Lorette Yimgang
,
Julius Nsami Ndi
* ORCID logo ,
Paul Keilah Lunga
* ORCID logo ,
Fabrice Fekam Boyom
ORCID logo
Version 1 : Received: 24 October 2023 / Approved: 25 October 2023 / Online: 25 October 2023 (09:31:02 CEST)

A peer-reviewed article of this Preprint also exists.

Tchinda Taghu, F.L.; Pone Kamdem, B.; Ngouana, V.; Yajeh Tanka, Z.; Yimgang, V.L.; Nsami Ndi, J.; Keilah Lunga, P.; Fekam Boyom, F. Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies. Drugs Drug Candidates 2024, 3, 13-32. Tchinda Taghu, F.L.; Pone Kamdem, B.; Ngouana, V.; Yajeh Tanka, Z.; Yimgang, V.L.; Nsami Ndi, J.; Keilah Lunga, P.; Fekam Boyom, F. Biological Synthesis and Characterization of Silver-Doped Nanocomposites: Antibacterial and Mechanistic Studies. Drugs Drug Candidates 2024, 3, 13-32.

Abstract

In this study, we report the antibacterial mechanisms of action of uniform silver nanoparticles (AgNPs) and decorated activated carbon nanocomposite (CAC-AgNPs) obtained using a green synthesis approach. The nanomaterials were characterized by ultraviolet-visible (UV-vis) absorption spectra and Fourier transform infrared (FTIR) spectra. The antibacterial activity of the as-prepared nanomaterials was evaluated against an array of bacterial strains by microdilution method, whereas their cytotoxicity profile was evaluated on Vero cells (human mammalian cells). The antibacterial mechanistic studies of active nanomaterials were carried out through bacterial growth kinetics, nucleic acid leakage test, and catalase inhibition assay. A silver nanocomposite was successfully fabricated from Croton macrostachyus-based activated carbon. The as-prepared nanomaterials exhibited antibacterial activity against an array of bacterial strains (minimum inhibitory concentration (MIC) range: 62.5 to 500 µg/mL), the most susceptible being Escherichia coli and Staphylococcus aureus. Cytotoxicity studies of the nanomaterials on Vero cells revealed that the nanocomposite (median cytotoxic concentration (CC50): 213.6 µg/mL) was less toxic than the nanoparticles (CC50 value: 164.75 µg/mL) counterpart. Antibacterial mechanistic studies unveiled that the nanomaterials induced (i) bacteriostatic activity vis-à-vis E. coli and S. aureus and (ii) inhibition of catalase in these bacteria. This novel contribution on the antibacterial mechanisms of action of silver nanocomposite from C. macrostachyus-based activated carbon might contribute to the understanding of antibacterial action of these biomaterials. Nevertheless, more chemistry and in vivo experiments, as well as in depth antibacterial mechanistic studies are warranted for the successful utilization of these antibacterial biomaterials.

Keywords

silver nanomaterials; activated carbon; infectious diseases; antibacterial mechanism of action; cytotoxicity

Subject

Medicine and Pharmacology, Pharmacology and Toxicology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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