PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Transcriptome Analysis Reveals the Essential Role of the Domesticated Gag Gene of Drosophila LTR Retrotransposons, Gagr, in the Modulation of Immune Response
Version 1
: Received: 22 October 2023 / Approved: 22 October 2023 / Online: 24 October 2023 (08:35:20 CEST)
How to cite:
Balakireva, Y.; Nikitina, M.; Makhnovskii, P.; Kukushkina, I.; Kuzmin, I.; Kim, A.; Nefedova, L. Transcriptome Analysis Reveals the Essential Role of the Domesticated Gag Gene of Drosophila LTR Retrotransposons, Gagr, in the Modulation of Immune Response. Preprints2023, 2023101510. https://doi.org/10.20944/preprints202310.1510.v1
Balakireva, Y.; Nikitina, M.; Makhnovskii, P.; Kukushkina, I.; Kuzmin, I.; Kim, A.; Nefedova, L. Transcriptome Analysis Reveals the Essential Role of the Domesticated Gag Gene of Drosophila LTR Retrotransposons, Gagr, in the Modulation of Immune Response. Preprints 2023, 2023101510. https://doi.org/10.20944/preprints202310.1510.v1
Balakireva, Y.; Nikitina, M.; Makhnovskii, P.; Kukushkina, I.; Kuzmin, I.; Kim, A.; Nefedova, L. Transcriptome Analysis Reveals the Essential Role of the Domesticated Gag Gene of Drosophila LTR Retrotransposons, Gagr, in the Modulation of Immune Response. Preprints2023, 2023101510. https://doi.org/10.20944/preprints202310.1510.v1
APA Style
Balakireva, Y., Nikitina, M., Makhnovskii, P., Kukushkina, I., Kuzmin, I., Kim, A., & Nefedova, L. (2023). Transcriptome Analysis Reveals the Essential Role of the Domesticated Gag Gene of Drosophila LTR Retrotransposons, Gagr, in the Modulation of Immune Response. Preprints. https://doi.org/10.20944/preprints202310.1510.v1
Chicago/Turabian Style
Balakireva, Y., Alexander Kim and Lidia Nefedova. 2023 "Transcriptome Analysis Reveals the Essential Role of the Domesticated Gag Gene of Drosophila LTR Retrotransposons, Gagr, in the Modulation of Immune Response" Preprints. https://doi.org/10.20944/preprints202310.1510.v1
Abstract
Background: The molecular domestication of the gag gene of retrotransposons and retroviruses gave rise to the Gagr gene in the genome of Drosophila. The Gagr protein has a conservative structure in all Drosophila species, suggesting an essential function. As we previously shown, the Gagr gene may play a part in immune response and processes linked to stress reactions. Methods: Tub-GAL4>UAS-Gagr flies, which had the Gagr gene knockdown in all tissues, were compared with the control hybrid Tub-GAL4>w1118. Gagr gene function was verified by RNA-sequencing followed by RT-PCR and physiological tests. Results: In contrast to the control strain, we observed that flies with the Gagr gene knockdown had a shorter lifespan, but the mutant strain was more resistant to heat stress. Also, the Gagr knockdown strain had higher level of transcription of the immune response genes, according to a transcriptome analysis. It has been shown that the ammonium persulfate used to induce stress causes the Toll, Jal-STAT, and Jnk/MAPK signaling pathways to become activated, which results in a systemic response in numerus tissues in the control strain. Conversely, the Gagr gene mutant strain exhibits low expression of the stress response. Enrichment of the molecular function of genes overexpressed under ammonium persulfate stress in the control strain, but not in the Gagr knockdown mutant, revealed a category with 19 transcription factors involved in the control of organism development, morphogenesis and the functioning of the central nervous system. Their expression pattern and the Gagr gene's expression pattern match. The data obtained demonstrates the importance Gagr is to maintaining both the body's immune system and homeostasis.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
