Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chemical Composition, Antioxidant and Anticancer Activity of Pericarp Water Extracts of Mediterranean Ancient Chestnut Accessions

Ylenia Spissu
ORCID logo ,
Maria Giovanna Molinu
* ,
Guy D’hallewin
* ,
Giulia Sanna
,
Gavina Rita Serra
,
Antonella Muroni
,
Antonio Barberis
ORCID logo
Version 1 : Received: 20 October 2023 / Approved: 23 October 2023 / Online: 23 October 2023 (09:56:13 CEST)

How to cite: Spissu, Y.; Molinu, M.G.; D’hallewin, G.; Sanna, G.; Serra, G.R.; Muroni, A.; Barberis, A. Chemical Composition, Antioxidant and Anticancer Activity of Pericarp Water Extracts of Mediterranean Ancient Chestnut Accessions. Preprints 2023, 2023101403. https://doi.org/10.20944/preprints202310.1403.v1 Spissu, Y.; Molinu, M.G.; D’hallewin, G.; Sanna, G.; Serra, G.R.; Muroni, A.; Barberis, A. Chemical Composition, Antioxidant and Anticancer Activity of Pericarp Water Extracts of Mediterranean Ancient Chestnut Accessions. Preprints 2023, 2023101403. https://doi.org/10.20944/preprints202310.1403.v1

Abstract

The residue of chestnut processing generates a large amount of waste material, a resource not adequately exploited. The phenolic composition of water extracts from discarded pericarp of four (MURG, LOCG, ILDP and COEV) chestnut accessions and one marron variety belonging to the Sardinian biodiversity was studied. The antioxidant capacity of cold and hot water extracts was determined by DPPH, ABTS and cyclic voltammetry tests. The antiproliferative effect on normal cells (fibroblasts), and colon (RKO and SW48), breast (MCF7) and melanoma (B16F10) cancer cells, was evaluated by biological assays. MTT test demonstrated that temperature and different extraction times significantly influenced the growth of cells, both normal and tumor. The fibroblast viability was significantly reduced by moderate doses of cold extracts, but only by doses greater than 250 µg/ml of hot extracts, regardless of the accession or cultivar. An even more marked effect was observed when RKO and SW48 were treated with cold extracts, while treatments on B16F10 melanoma cells resulted less effective. Differently, the cold extracts of all accessions induced a significant increase in MCF7 cell viability starting from moderate doses (50 µg/ml), whereas hot extracts had a similar, but less evident, effect compared to reference fibroblasts.

Keywords

Chestnut biodiversity; pericarp; water extraction; antioxidant activity; cancer cells

Subject

Public Health and Healthcare, Other

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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