Version 1
: Received: 9 October 2023 / Approved: 10 October 2023 / Online: 10 October 2023 (10:09:46 CEST)
How to cite:
Medina-Ramirez, I.E.; Macías-Díaz, J.E.; Masuoka, D.; Zapien, J.A. Holotomography and Atomic Force Microscopy: A Powerful Combination to Enhance Cancer, Microbiology and Nanotoxicology Research. Preprints2023, 2023100615. https://doi.org/10.20944/preprints202310.0615.v1
Medina-Ramirez, I.E.; Macías-Díaz, J.E.; Masuoka, D.; Zapien, J.A. Holotomography and Atomic Force Microscopy: A Powerful Combination to Enhance Cancer, Microbiology and Nanotoxicology Research. Preprints 2023, 2023100615. https://doi.org/10.20944/preprints202310.0615.v1
Medina-Ramirez, I.E.; Macías-Díaz, J.E.; Masuoka, D.; Zapien, J.A. Holotomography and Atomic Force Microscopy: A Powerful Combination to Enhance Cancer, Microbiology and Nanotoxicology Research. Preprints2023, 2023100615. https://doi.org/10.20944/preprints202310.0615.v1
APA Style
Medina-Ramirez, I.E., Macías-Díaz, J.E., Masuoka, D., & Zapien, J.A. (2023). Holotomography and Atomic Force Microscopy: A Powerful Combination to Enhance Cancer, Microbiology and Nanotoxicology Research. Preprints. https://doi.org/10.20944/preprints202310.0615.v1
Chicago/Turabian Style
Medina-Ramirez, I.E., David Masuoka and Juan Antonio Zapien. 2023 "Holotomography and Atomic Force Microscopy: A Powerful Combination to Enhance Cancer, Microbiology and Nanotoxicology Research" Preprints. https://doi.org/10.20944/preprints202310.0615.v1
Abstract
Modern imaging strategies are paramount to studying living systems such as cells, bacteria, and fungi and their response to pathogens, toxicants, and nanomaterials as modulated by exposure and environmental factors. The need to understand the processes and mechanisms of damage, healing and cell survivability of living systems continues to motivate the development of alternative imaging strategies. Of particular interest is the use of label-free techniques that minimize interference of biological processes by foreign marking substances and reduce intense light exposure and potential photo-toxicity effects. This review focus on the potential synergic capabilities of atomic force microscopy (AFM) as a well-developed and robust imaging strategy with demonstrated applications to unravel intimate details in biomedical applications, with the label-free, fast, and enduring Holotomographic Microscopy (HT) strategy. We first review the operating principles that form the basis for the complementary details provided by these techniques regarding the surface and internal information provided by HT and AFM is essential and complimentary for the development of several biomedical areas studying the interaction mechanisms of nanomaterials with living organisms. First, AFM can provide superb resolution on surface morphology and biomechanical characterization. Second, the quantitative phase capabilities of HT enable superb modeling and quantification of the volume, surface area, protein content, and mass density of the main components of cells and microorganisms, including morphology of cells in microbiological systems. These capabilities result from directly quantifying refractive index changes without requiring fluorescent markers or chemicals. As such, HT is ideally suited for long-term monitoring of living organisms in conditions close to their natural settings. We present a case-based review of the principal uses of both techniques and their essential contributions to nanomedicine and nanotoxicology, emphasizing cancer and infectious disease control. The synergic impact of the sequential use of these complementary strategies provides a clear drive for adopting these techniques as interdependent fundamental tools.
Keywords
Holotomography, Atomic force microscopy, Lable-free, Nanomedicine, Nanotoxicology, Refractive index.
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
