Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Evaluation of Cell Rupture Techniques on the Extraction of Proteins from Microalgae Tetradesmus obliquus

César Augusto Sodré da Silva
ORCID logo ,
Karen Vanessa Marimón Sibaja
,
Sabrina de Ramos Cizilio
,
José Roberto Miranda Júnior
ORCID logo ,
Rejane de Castro Santana
,
Marcio Arêdes Martins
ORCID logo ,
Maurício de Oliveira Leite
,
Eduardo Basílio de Oliveira
* ORCID logo ,
Jane Sélia dos Reis Coimbra
* ORCID logo
Version 1 : Received: 6 October 2023 / Approved: 6 October 2023 / Online: 9 October 2023 (08:56:51 CEST)

A peer-reviewed article of this Preprint also exists.

da Silva, C.A.S.; Sibaja, K.V.M.; de Ramos Cizilio, S.; Miranda Júnior, J.R.; de Castro Santana, R.; Martins, M.A.; de Oliveira Leite, M.; de Oliveira, E.B.; Coimbra, J.S.R. Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus. Phycology 2024, 4, 87-103. da Silva, C.A.S.; Sibaja, K.V.M.; de Ramos Cizilio, S.; Miranda Júnior, J.R.; de Castro Santana, R.; Martins, M.A.; de Oliveira Leite, M.; de Oliveira, E.B.; Coimbra, J.S.R. Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus. Phycology 2024, 4, 87-103.

Abstract

The high protein content of several microalgal species makes them attractive and unconventional for applications in the food and pharmaceutical industries. Due to microalgae's robust cell wall, cell rupture is necessary to improve the extraction of their intracellular proteins. Thus, choosing a suitable cell-breaking treatment before protein extraction is a vital downstream processing step. Additionally, it is necessary to use an effective technique for monitoring and measuring the impact of rupture treatments on microalgal cell walls. In our study, Tetradesmus obliquus cells were disrupted by three different mechanical rupture methods: high-pressure homogenization (HPH), ultrasound (US), and ball milling (BM). The ruptured biomass cells were counted, and soluble proteins were extracted and quantified. The cell counting technique did not detect differences between intact and damaged cells after BM treatment because the dye (erythrosine B) did not permeate the microalgae biomass accurately. The US treatment promoted the highest yield of total protein extraction (19.95%). The yields of protein extraction of T. obliquus with HPH (15.68%) and BM (14.11%) were significantly (p value ≤ 0.05) lower than that achieved in the US treatment. The increase in the number of extraction steps increased the protein recovery. Thus, the content of extracted soluble protein was a reliable indirect index for a quantitative comparison of the cell disruption level.

Keywords

ball milling; high-pressure homogenization; ultrasonication; cell counting

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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