MSC Sheet Assembly Duration Correlates with Extracellular Matrix Deposition, but Not Proliferative Activity or Donor Features

Valentina Sergeevna Glazyeva; Natalya Andreevna Alexandrushkina; Peter Petrovich Nimiritsky; Maria Aleksandrovna Kulebyakina; Roman Yurievich Eremichev; Pavel Igorevich Makarevich

doi:10.20944/preprints202310.0092.v1

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preprints.org > biology and life sciences > cell and developmental biology > doi: 10.20944/preprints202310.0092.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

MSC Sheet Assembly Duration Correlates with Extracellular Matrix Deposition, but Not Proliferative Activity or Donor Features

Valentina Sergeevna Glazyeva

Natalya Andreevna Alexandrushkina

Peter Petrovich Nimiritsky

Maria Aleksandrovna Kulebyakina

Roman Yurievich Eremichev

Pavel Igorevich Makarevich

Version 1 : Received: 2 October 2023 / Approved: 2 October 2023 / Online: 3 October 2023 (09:15:33 CEST)

A peer-reviewed article of this Preprint also exists.

Glazieva, V.S.; Alexandrushkina, N.A.; Nimiritsky, P.P.; Kulebyakina, M.A.; Eremichev, R.Y.; Makarevich, P.I. Extracellular Matrix Deposition Defines the Duration of Cell Sheet Assembly from Human Adipose-Derived MSC. Int. J. Mol. Sci. 2023, 24, 17050. Glazieva, V.S.; Alexandrushkina, N.A.; Nimiritsky, P.P.; Kulebyakina, M.A.; Eremichev, R.Y.; Makarevich, P.I. Extracellular Matrix Deposition Defines the Duration of Cell Sheet Assembly from Human Adipose-Derived MSC. Int. J. Mol. Sci. 2023, 24, 17050.

Abstract

Cell sheet (CS) engineering using human MSC is of significant interest for regenerative medicine and this rapidly growing field translates to clinical use in a number of indications. Nevertheless, little is known of factors that define the timing of CS assembly from primary cultures which is important for planning treatments in both – autologous and allogeneic modes of use. We used a comparative in vitro approach with primary donors’ (n=14) adipose-derived MSC and evaluated the impact of healthy subject’s characteristics (age and sex), MSC culture features (population doubling time and lag-phase) and extracellular matrix (ECM) composition along with factors related to connective tissue formations (-SMA and FAP-) on CS assembly duration.

Keywords

cell sheet; tissue engineering; mesenchymal stromal cells; extracellular matrix; fibroblast activating protein; myofibroblast

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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