Version 1
: Received: 27 September 2023 / Approved: 28 September 2023 / Online: 28 September 2023 (18:06:19 CEST)
How to cite:
Lee, Y.; Kim, M.; Kim, H.; Kang, J.L. Administration of Gas6 Attenuates Lung Fibrosis via Inhibition of the Epithelial-Mesenchymal Transition and Fibroblast Activation. Preprints2023, 2023091996. https://doi.org/10.20944/preprints202309.1996.v1
Lee, Y.; Kim, M.; Kim, H.; Kang, J.L. Administration of Gas6 Attenuates Lung Fibrosis via Inhibition of the Epithelial-Mesenchymal Transition and Fibroblast Activation. Preprints 2023, 2023091996. https://doi.org/10.20944/preprints202309.1996.v1
Lee, Y.; Kim, M.; Kim, H.; Kang, J.L. Administration of Gas6 Attenuates Lung Fibrosis via Inhibition of the Epithelial-Mesenchymal Transition and Fibroblast Activation. Preprints2023, 2023091996. https://doi.org/10.20944/preprints202309.1996.v1
APA Style
Lee, Y., Kim, M., Kim, H., & Kang, J.L. (2023). Administration of Gas6 Attenuates Lung Fibrosis via Inhibition of the Epithelial-Mesenchymal Transition and Fibroblast Activation. Preprints. https://doi.org/10.20944/preprints202309.1996.v1
Chicago/Turabian Style
Lee, Y., Hee-Sun Kim and Jihee Lee Kang. 2023 "Administration of Gas6 Attenuates Lung Fibrosis via Inhibition of the Epithelial-Mesenchymal Transition and Fibroblast Activation" Preprints. https://doi.org/10.20944/preprints202309.1996.v1
Abstract
The epithelial-mesenchymal transition (EMT) is a major event in idiopathic pulmonary fibrosis pathogenesis. Here, we investigated whether growth arrest-specific protein 6 (Gas6) plays a protective role in lung fibrosis via suppression of the EMT and fibroblast activation. rGas6 ad-ministration inhibited the EMT in isolated mouse ATII cells 14 days post-BLM treatment based on morphologic cellular alterations, changes in mRNA and protein expression profiles of EMT markers, and induction of EMT-activating transcription factors. BLM-induced increases in gene expression of fibroblast activation-related markers and the invasive capacity of primary lung fi-broblasts in primary lung fibroblasts were reversed by rGas6 administration. Furthermore, the hydroxyproline content and collagen accumulation in interstitial areas with damaged alveolar structures in lung tissue were reduced by rGas6 administration. Targeting Gas6/Axl signaling events with specific inhibitors of Axl (BGB324), COX-2 (NS-398), EP1/EP2 receptor (AH-6809), or PGD2 DP2 receptor (BAY-u3405) reversed the inhibitory effects of rGas6 on EMT and fibroblast activation. Finally, we confirmed the antifibrotic effects of Gas6 using Gas6−/− mice. Therefore, Gas6/Axl signaling events play a potential role in inhibition of EMT process and fibroblast acti-vation via COX-2-derived PGE2 and PGD2 production, ultimately preventing the development of pulmonary fibrosis.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
