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Integration of Metabolomics and Transcriptomics to Explore Dynamic Alterations in Fruit Color and Quality in ‘Comte De Paris’ Pineapple during Ripening Processes
Song, K.; Zhang, X.; Liu, J.; Yao, Q.; Li, Y.; Hou, X.; Liu, S.; Qiu, X.; Yang, Y.; Chen, L.; Hong, K.; Lin, L. Integration of Metabolomics and Transcriptomics to Explore Dynamic Alterations in Fruit Color and Quality in ‘Comte de Paris’ Pineapples during Ripening Processes. Int. J. Mol. Sci.2023, 24, 16384.
Song, K.; Zhang, X.; Liu, J.; Yao, Q.; Li, Y.; Hou, X.; Liu, S.; Qiu, X.; Yang, Y.; Chen, L.; Hong, K.; Lin, L. Integration of Metabolomics and Transcriptomics to Explore Dynamic Alterations in Fruit Color and Quality in ‘Comte de Paris’ Pineapples during Ripening Processes. Int. J. Mol. Sci. 2023, 24, 16384.
Song, K.; Zhang, X.; Liu, J.; Yao, Q.; Li, Y.; Hou, X.; Liu, S.; Qiu, X.; Yang, Y.; Chen, L.; Hong, K.; Lin, L. Integration of Metabolomics and Transcriptomics to Explore Dynamic Alterations in Fruit Color and Quality in ‘Comte de Paris’ Pineapples during Ripening Processes. Int. J. Mol. Sci.2023, 24, 16384.
Song, K.; Zhang, X.; Liu, J.; Yao, Q.; Li, Y.; Hou, X.; Liu, S.; Qiu, X.; Yang, Y.; Chen, L.; Hong, K.; Lin, L. Integration of Metabolomics and Transcriptomics to Explore Dynamic Alterations in Fruit Color and Quality in ‘Comte de Paris’ Pineapples during Ripening Processes. Int. J. Mol. Sci. 2023, 24, 16384.
Abstract
To understand the molecular mechanism underlying yellowing in pineapples during ripening, coupled with alterations in fruit quality, comprehensive metabolome and transcriptome investigations were carried out. These investigations were conducted using the pulp samples collected at three distinct stages of maturity: young fruit (YF), mature fruit (MF), and fully mature fruit (FMF). This study revealed a noteworthy increase in the levels of total phenols and flavones, coupled with a concurrent decline in lignin and total acid content, as the fruit transitioned from YF to FMF. Furthermore, the analysis yielded 167 differentially accumulated metabolites (DAMs) and 2194 differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes enrichment analysis based on DAMs and DEGs revealed that the biosynthesis of plant secondary metabolites, particularly the flavonol, flavonoid, and phenypropanoid pathways, plays a pivotal role in fruit yellowing. Additionally, a comprehensive regulatory network encompassing genes that contribute to metabolisms of flavones, flavonols, lignin, and organic acids was proposed. This network sheds light on the intricate processes that underlies fruit yellowing and quality alterations. These findings enhance our understanding of the regulatory pathways governing pineapple ripening and offer valuable scientific insight into the molecular breeding of pineapples.
Keywords
pineapple; ripening; yellowing; fruit quality; metabolomics; transcriptomics
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
