Version 1
: Received: 21 September 2023 / Approved: 22 September 2023 / Online: 25 September 2023 (12:34:21 CEST)
How to cite:
Yeboah, P.J.; Eddin, A.S.; Wijemanna, N.D.; Williams, L.L.; Krastanov, A.; Ibrahim, S.A. Optimization of Plant-Based Medium for Growth and Viability of Lactobacillus delbrueckii ssp. bulgaricus. Preprints2023, 2023091574. https://doi.org/10.20944/preprints202309.1574.v1
Yeboah, P.J.; Eddin, A.S.; Wijemanna, N.D.; Williams, L.L.; Krastanov, A.; Ibrahim, S.A. Optimization of Plant-Based Medium for Growth and Viability of Lactobacillus delbrueckii ssp. bulgaricus. Preprints 2023, 2023091574. https://doi.org/10.20944/preprints202309.1574.v1
Yeboah, P.J.; Eddin, A.S.; Wijemanna, N.D.; Williams, L.L.; Krastanov, A.; Ibrahim, S.A. Optimization of Plant-Based Medium for Growth and Viability of Lactobacillus delbrueckii ssp. bulgaricus. Preprints2023, 2023091574. https://doi.org/10.20944/preprints202309.1574.v1
APA Style
Yeboah, P.J., Eddin, A.S., Wijemanna, N.D., Williams, L.L., Krastanov, A., & Ibrahim, S.A. (2023). Optimization of Plant-Based Medium for Growth and Viability of <i>Lactobacillus delbrueckii </i>ssp.<i> bulgaricus</i>. Preprints. https://doi.org/10.20944/preprints202309.1574.v1
Chicago/Turabian Style
Yeboah, P.J., Albert Krastanov and Salam A. Ibrahim. 2023 "Optimization of Plant-Based Medium for Growth and Viability of <i>Lactobacillus delbrueckii </i>ssp.<i> bulgaricus</i>" Preprints. https://doi.org/10.20944/preprints202309.1574.v1
Abstract
The objective of the present study was to develop and optimize an efficient growth media for Lactobacillus delbrueckii ssp. bulgaricus, an essential dairy starter culture, uses plant-based nitrogen sources. The composition of the de Man, Rogosa, and Sharpe (MRS) culture medium was modified, and the nitrogen content was replaced by alternative nitrogen sources X-Seed N7 and N5 in various blends of 3% and 1.5%. Results showed that bacterial growth was higher with the N7 and N5 nitrogen source blends at 3% and 1.5%. Similarly, the optical densities of the L. bulgaricus strains were higher in the N7 and N5 blends (1.5/3%) compared to the MRS medium. Although there was no significance in bacterial counts for both the MRS and the N7 and N5 media, a significant difference between the control medium and the optimized growth media was noted. Our results thus suggest that the alternative nitrogen sources X-Seed N7 and N5 can substantially support the growth of lactic acid bacteria as shown with L. bulgaricus. Moreover, the presence of complex nitrogen sources rich in free nucleotides, amino acids, and peptides enhanced L. bulgaricus growth. Our results support the development of cost-effective and plant-based growth media for LAB cultures and potential benefits to the food and dairy industries. Additional research is warranted to improve buffering capacity for higher cell counts.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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