Version 1
: Received: 20 September 2023 / Approved: 21 September 2023 / Online: 21 September 2023 (12:16:03 CEST)
How to cite:
Almusined, M.; Kakkar, P.; Yuldasheva, N.Y.; Viswambharan, H.; Kakkar, T.; Saha, S. Investigation of Blood-Brain Barrier proteins as Potential Biomarkers in a Murine Model of Ischaemic Stroke. Preprints2023, 2023091491. https://doi.org/10.20944/preprints202309.1491.v1
Almusined, M.; Kakkar, P.; Yuldasheva, N.Y.; Viswambharan, H.; Kakkar, T.; Saha, S. Investigation of Blood-Brain Barrier proteins as Potential Biomarkers in a Murine Model of Ischaemic Stroke. Preprints 2023, 2023091491. https://doi.org/10.20944/preprints202309.1491.v1
Almusined, M.; Kakkar, P.; Yuldasheva, N.Y.; Viswambharan, H.; Kakkar, T.; Saha, S. Investigation of Blood-Brain Barrier proteins as Potential Biomarkers in a Murine Model of Ischaemic Stroke. Preprints2023, 2023091491. https://doi.org/10.20944/preprints202309.1491.v1
APA Style
Almusined, M., Kakkar, P., Yuldasheva, N.Y., Viswambharan, H., Kakkar, T., & Saha, S. (2023). Investigation of Blood-Brain Barrier proteins as Potential Biomarkers in a Murine Model of Ischaemic Stroke. Preprints. https://doi.org/10.20944/preprints202309.1491.v1
Chicago/Turabian Style
Almusined, M., Tarun Kakkar and Sikha Saha. 2023 "Investigation of Blood-Brain Barrier proteins as Potential Biomarkers in a Murine Model of Ischaemic Stroke" Preprints. https://doi.org/10.20944/preprints202309.1491.v1
Abstract
The blood-brain barrier (BBB) acts as a specialized structure separating the brain from peripheral blood circulation and plays an important role in brain function. Following an ischaemic stroke or cerebral ischaemia, the BBB is damaged leading to degraded proteins being released into blood circulation. However, little is known about cerebral ischaemia and reperfusion (I/R) induced BBB damage and changes in circulatory biomarkers. This study aims to use both immunohistochemistry and western blotting (WB) to examine neuronal death, glial cell alterations and changes in BBB tight junction (TJ) proteins such as zonula occludens-1 (ZO-1), Occludin and Claudin-5 in the hippocampus in a murine model of cerebral I/R. The changes in these proteins in the blood serum of this model were assessed by enzyme-linked immunosorbent assay. The results showed neuronal death and a significant increase in glial fibrillary acidic protein (GFAP), a protein primarily expressed in astrocytes and a significant decrease in TJ proteins, ZO-1, Occludin and Claudin-5 in the hippocampus of occluded mice as compared to sham-operated mice. These changes are associated with an increased level of these proteins in blood serum in ischaemic mice, suggesting that these proteins can be used as potential biomarkers for determining ischaemic stroke.
Keywords
Blood-brain barrier; Brain ischaemia; Stroke; Biomarker; Tight Junction protein
Subject
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
