Version 1
: Received: 21 September 2023 / Approved: 21 September 2023 / Online: 21 September 2023 (07:42:37 CEST)
How to cite:
Ariski, R.T.; Lee, K.K.; Kim, Y.; Lee, C. The Impact of pH and Temperature on the Green Gold Nanoparticles Preparation Using Jeju Hallabong Peel Extract for Biomedical Applications. Preprints2023, 2023091459. https://doi.org/10.20944/preprints202309.1459.v1
Ariski, R.T.; Lee, K.K.; Kim, Y.; Lee, C. The Impact of pH and Temperature on the Green Gold Nanoparticles Preparation Using Jeju Hallabong Peel Extract for Biomedical Applications. Preprints 2023, 2023091459. https://doi.org/10.20944/preprints202309.1459.v1
Ariski, R.T.; Lee, K.K.; Kim, Y.; Lee, C. The Impact of pH and Temperature on the Green Gold Nanoparticles Preparation Using Jeju Hallabong Peel Extract for Biomedical Applications. Preprints2023, 2023091459. https://doi.org/10.20944/preprints202309.1459.v1
APA Style
Ariski, R.T., Lee, K.K., Kim, Y., & Lee, C. (2023). The Impact of pH and Temperature on the Green Gold Nanoparticles Preparation Using Jeju Hallabong Peel Extract for Biomedical Applications. Preprints. https://doi.org/10.20944/preprints202309.1459.v1
Chicago/Turabian Style
Ariski, R.T., YongKwan Kim and Chang-Soo Lee. 2023 "The Impact of pH and Temperature on the Green Gold Nanoparticles Preparation Using Jeju Hallabong Peel Extract for Biomedical Applications" Preprints. https://doi.org/10.20944/preprints202309.1459.v1
Abstract
The utilization of gold nanoparticles (AuNPs) has garnered significant attention in recent times, particularly in the field of biomedical research. The utilization of AuNPs in chemical synthesis procedures raises apprehensions regarding their potential toxicity in living organisms, which is inconsistent with their purported eco-friendly and cost-effective aspects. In this investigation, AuNPs were synthesized via the green synthesis approach utilizing Jeju Hallabong peel extract (HPE), a typical fruit variety indigenous to South Korea. The visible-range absorption spectrum of gold nanoparticles from green synthesis (HAuNPs) that are red wine in color occurs at a wavelength of λ = 517 nm. The morphology and particle size distribution were analysed using transmission electron microscopy (TEM) and ImageJ software. The TEM images reveal that the HAuNPs exhibit a high degree of dispersion and uniformity in their spherical shape, with an average size of approximately 7 nm. Moreover, elevating the initial pH level of the mixed solution has an impact on the decrease in particle dimensions, as evidenced by the blue shift observed in the UV-visible spectroscopy absorbance peak. Elevating the reaction temperature may accelerate the synthesis duration. However, it does not exert a substantial impact on the particle dimensions. The outcomes of an avidin-biocytin colorimetric assay provide preliminary analyses of possible sensor tunability using HAuNPs. The cytotoxicity of HAuNPs was evaluated through in vitro studies using the MTT assay on RAW 264.7 cell lines. The results indicated that the HAuNPs exhibited lower cytotoxicity compared to both chemically reduced gold nanoparticles (CAuNPs) and commercially available gold nanoparticles (SAAuNPs).
Keywords
gold nanoparticles; Hallabong peel extract; green synthesis; colorimetric; cytotoxicity
Subject
Chemistry and Materials Science, Materials Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
