Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?

Aleksandr R. Shevchenko
ORCID logo ,
Ksenia A. Mayorova
ORCID logo ,
Dmitry G. Chukhchin
,
Alexey V. Malkov
,
Evgeniy A. Toptunov
ORCID logo ,
Vadim D. Telitsin
,
Aleksandra M. Rozhkova
,
Ivan N. Zorov
,
Maria A. Rodicheva
,
Vadim A. Plakhin
,
Denis A. Akishin
,
Daria N. Poshina
ORCID logo ,
Margarita V. Semenova
,
Andrey S. Aksenov
* ,
Arkady Sinitsyn
Version 1 : Received: 18 September 2023 / Approved: 19 September 2023 / Online: 20 September 2023 (08:02:44 CEST)

A peer-reviewed article of this Preprint also exists.

Shevchenko, A.R.; Mayorova, K.A.; Chukhchin, D.G.; Malkov, A.V.; Toptunov, E.A.; Telitsin, V.D.; Rozhkova, A.M.; Zorov, I.N.; Rodicheva, M.A.; Plakhin, V.A.; Akishin, D.A.; Poshina, D.N.; Semenova, M.V.; Aksenov, A.S.; Sinitsyn, A.P. Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification? Fermentation 2023, 9, 936. Shevchenko, A.R.; Mayorova, K.A.; Chukhchin, D.G.; Malkov, A.V.; Toptunov, E.A.; Telitsin, V.D.; Rozhkova, A.M.; Zorov, I.N.; Rodicheva, M.A.; Plakhin, V.A.; Akishin, D.A.; Poshina, D.N.; Semenova, M.V.; Aksenov, A.S.; Sinitsyn, A.P. Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification? Fermentation 2023, 9, 936.

Abstract

Sulfite and kraft pulping are two principal methods of industrial delignification of wood. In recent decades, those have been considered as possibilities to pretreat recalcitrant wood lignocellulosics for enzymatic hydrolysis of polysaccharides and subsequent fermentation of obtained sugars to valuable bioproducts. Current work compares chemistry and technological features of two different cooking processes in the preparation of polysaccharide substrates for deep sacchari-fication with P. verruculosum glycosyl hydrolases. Bleached kraft and sulfite pulps were subjected to hydrolysis with enzyme mixture of high xylanase, cellobiohydrolase and β-glucosidase activities at a dosage of 10 FPU/g of dry pulp and fiber concentration of 2.5, 5 and 10%. HPLC was used to analyze soluble sugars after hydrolysis and additional acid inversion of oligomers to monosaccharides. Kraft pulp demonstrated higher pulp conversion after 48 h (74-99%), mostly resulted from deep xylan hydrolysis. Sulfite-pulp hydrolysates, obtained in similar conditions, due to higher hexose concentration (more than 50 g/L), had higher fermentability for industrial strains producing alcohols, microbial protein or organic acids. Along with saccharification, enzymatic modification of non-hydrolysed residues occurred, which led to decreased degree of polymerization and composition changes in two industrial pulps. As a result, crystallinity of kraft pulp increased by 1.3%, which opens possibilities for obtaining new types of cellulosic products in pulp and paper industry. The high adaptability and controllability of enzymatic and fermentation processes creates prospects for the modernization of existing factories.

Keywords

wood lignocellulose; pretreatment; enzymatic saccharification; glucose; kraft pulping; sulfite delignification; biomodified pulp

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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