Multiplex Portable Biosensor for Bacteria Detection

Estefania Enebral- Romero; Karim Kaci; Emiliano Martinez-Periñan; Marina Garrido; Emilio Pérez; David López-Diego; Monica Luna; Guillermo González-de-Rivera; Tania García-Mendiola; Encarnación Lorenzo

doi:10.20944/preprints202309.0942.v1

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preprints.org > chemistry and materials science > analytical chemistry > doi: 10.20944/preprints202309.0942.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Multiplex Portable Biosensor for Bacteria Detection

Estefania Enebral- Romero

Karim Kaci

Emiliano Martinez-Periñan

Guillermo González-de-Rivera

Tania García-Mendiola

^* ,

Encarnación Lorenzo

Version 1 : Received: 12 September 2023 / Approved: 13 September 2023 / Online: 14 September 2023 (08:53:53 CEST)

A peer-reviewed article of this Preprint also exists.

Kaci, K.; Enebral-Romero, E.; Martínez-Periñán, E.; Garrido, M.; Pérez, E.M.; López-Diego, D.; Luna, M.; González-de-Rivera, G.; García-Mendiola, T.; Lorenzo, E. Multiplex Portable Biosensor for Bacteria Detection. Biosensors 2023, 13, 958. Kaci, K.; Enebral-Romero, E.; Martínez-Periñán, E.; Garrido, M.; Pérez, E.M.; López-Diego, D.; Luna, M.; González-de-Rivera, G.; García-Mendiola, T.; Lorenzo, E. Multiplex Portable Biosensor for Bacteria Detection. Biosensors 2023, 13, 958.

Abstract

An advanced, cost-effective and portable DNA biosensor capable of detecting multiple bacteria simultaneously have been developed. The biosensor consists of a fast and inexpensive potentiostat that controls the applied potential to a screen-printed electrochemical array platform functionalized with MoS2 flakes and bacteria DNA probes, and monitors the current response obtained by the use of à la carte thionine functionalized carbon nanodots (Ty-CDs) as electrochemical indicator of the hybridization event. The design of the potentiostat prioritizes achieving an optimal signal-to-noise ratio and incorporates a user-friendly interface compatible with various devices such as computers, mobile phones and tablets. The device is compact, lightweight, and manufactured at a low cost. The key components of the potentiostat include a data acquisition board capable of analyzing multiple samples simultaneously and a controller board. The results obtained confirm the ability of the developed device to successfully detect specific bacteria DNA sequences, demonstrating the reliability and performance of the developed multiplexing portable biosensor with better sensibility than using a traditional, more complex and laboratory oriented potentiostat.

Keywords

DNA biosensor, functionalized carbon nanodots, multiplex potentiostat, bacteria detection

Subject

Chemistry and Materials Science, Analytical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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