Cho, J.; Nouizi, F.; Kim, C.-S.; Gulsen, G. Monitoring Distribution of the Therapeutic Agent Dimethyl Sulfoxide via Solvatochromic Shift of Albumin-Bound Indocyanine Green. Sensors2023, 23, 7728.
Cho, J.; Nouizi, F.; Kim, C.-S.; Gulsen, G. Monitoring Distribution of the Therapeutic Agent Dimethyl Sulfoxide via Solvatochromic Shift of Albumin-Bound Indocyanine Green. Sensors 2023, 23, 7728.
Cho, J.; Nouizi, F.; Kim, C.-S.; Gulsen, G. Monitoring Distribution of the Therapeutic Agent Dimethyl Sulfoxide via Solvatochromic Shift of Albumin-Bound Indocyanine Green. Sensors2023, 23, 7728.
Cho, J.; Nouizi, F.; Kim, C.-S.; Gulsen, G. Monitoring Distribution of the Therapeutic Agent Dimethyl Sulfoxide via Solvatochromic Shift of Albumin-Bound Indocyanine Green. Sensors 2023, 23, 7728.
Abstract
We recently developed a novel hyperspectral excitation-resolved near-infrared fluorescence imaging system (HER-NIRF) based on a continuous-wave wavelength-swept laser. In this study, this technique is applied for measuring the distribution of the therapeutic agent dimethyl sulfoxide (DMSO) by utilizing solvatochromic shift in the spectral profile of albumin-bound Indocyanine green (ICG). Using wide-field imaging in turbid media, complex dynamics of albumin-bound ICG is measured in mixtures of dimethyl sulfoxide (DMSO) and water. Phantom experiments are conducted to evaluate the performance of the HER-NIRF system. The results show that the distribution of DMSO can be visualized in the wide-field reflection geometry. One of the main purposes of the DMSO is to act as a carrier for other drugs, enhancing their effects by facilitating skin penetration. Understanding solubility and permeability of drugs in vivo is very important in drug discovery and development. Hence, this HER-NIRF technique has a great potential to advance the utilization of the therapeutic agent DMSO by mapping its distribution via solvatochromic shift of ICG. By customizing the operational wavelength range, this system can be applied to any other fluorophores in the near infrared region and utilized for a wide variety of drug delivery studies.
Keywords
Fluorescence Imaging; Multispectral and hyperspectral imaging; Solvatochromic shift; Tunable Lasers; Spectroscopy; DMSO Sensing; Drug uptake monitoring
Subject
Engineering, Bioengineering
Copyright:
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