Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Andrographis paniculata Leaves Extract Alleviates UVB-induced HaCaT Cells through Suppressing MAPKs Activation

Al Borhan Bayazid
ORCID logo ,
Ue kyoung Hwang
,
Young-Ah Jang
ORCID logo ,
Yeong Hwan Jeong
,
Yu Chang Jo
,
Beong Ou Lim
* ORCID logo
Version 1 : Received: 30 August 2023 / Approved: 31 August 2023 / Online: 1 September 2023 (07:26:12 CEST)

A peer-reviewed article of this Preprint also exists.

Bayazid, A. B., Hwang, U. K., Jang, Y. A., Jeong, Y. H., Jo, Y. C., & Lim, B. O. (2024). Andrographis paniculata Leaves Extract Alleviates UVB-Induced HaCaT Cells Through Suppressing Mitogen-Activated Protein Kinases Activation. Natural Product Communications, 19(4). https://doi.org/10.1177/1934578X241238137 Bayazid, A. B., Hwang, U. K., Jang, Y. A., Jeong, Y. H., Jo, Y. C., & Lim, B. O. (2024). Andrographis paniculata Leaves Extract Alleviates UVB-Induced HaCaT Cells Through Suppressing Mitogen-Activated Protein Kinases Activation. Natural Product Communications, 19(4). https://doi.org/10.1177/1934578X241238137

Abstract

Andrographis paniculata leaves have been widely used in traditional medicine due to their rich content of phenolic compounds. In this study, we evaluated the total phenolic and flavonoid content of Andrographis paniculata leaf extracts (ALE), as well as its antioxidant activities against DPPH, ABTS, and hydrogen peroxide radicals. Additionally, we conducted cell viability assays, RT-PCR, and western blot analysis to investigate the biochemical effects of ALE on UVB-induced HaCaT cells. Our results revealed that ALE exhibited a high concentration of polyphenols and flavonoids, which were associated with potent abiotic antioxidant properties. Furthermore, ALE treatment effectively abrogated the damage caused by UVB radiation on key molecules such as hyaluronan synthase 1, Aquaporin 3, and loricrin in HaCaT cells. ALE also demonstrated the ability to reduce the overactivation of mitogen-activated protein kinases (MAPKs) and the expression of pro-inflammatory cytokines, including interleukin-6 and interleukin-1β in UVB-induced HaCaT cells. Additionally, ALE positively influenced the peroxisome proliferator-activated receptor alpha in these cells. Overall, our findings highlight the antioxidant and anti-inflammatory effects of ALE, as well as its significant role in modulating skin barrier functions. These results contribute to the growing body of evidence supporting the therapeutic potential of ALE in skincare and dermatological applications.

Keywords

Andrographis paniculate; MAPKs; interleukin; PPAR-α; skin barrier function

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.