Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Synergistic Antioxidant and Anti-Inflammatory Activities of Kale Juice Fermented with Limosilactobacills reuteri EFEL6901 or Limosilactobacills fermentum EFEL6800

Version 1 : Received: 30 August 2023 / Approved: 31 August 2023 / Online: 31 August 2023 (09:48:25 CEST)

A peer-reviewed article of this Preprint also exists.

Kim, G.Y.; Kim, S.-A.; Kong, S.Y.; Seong, H.; Bae, J.-H.; Han, N.S. Synergistic Antioxidant and Anti-Inflammatory Activities of Kale Juice Fermented with Limosilactobacills reuteri EFEL6901 or Limosilactobacills fermentum EFEL6800. Antioxidants 2023, 12, 1850. Kim, G.Y.; Kim, S.-A.; Kong, S.Y.; Seong, H.; Bae, J.-H.; Han, N.S. Synergistic Antioxidant and Anti-Inflammatory Activities of Kale Juice Fermented with Limosilactobacills reuteri EFEL6901 or Limosilactobacills fermentum EFEL6800. Antioxidants 2023, 12, 1850.

Abstract

This study investigates the synergistic impact of fermenting kale juice with Limosilactobacillus strains on its antioxidant and anti-inflammatory properties. Kale's rich nutrient profile, especially its flavonoids, offers potential health benefits. Probiotic lactic acid bacteria are employed in kale fermentation to enhance nutrient bioavailability and generate bioactive compounds. Kale juices fermented with L. reuteri EFEL6901 or L. fermentum EFEL6800 exhibited superior microbial growth. Free sugars and amino acids were converted to alcohols and organic acids, affecting the organoleptic and health-related properties of product. In addition, fermentation increased quercetin and kaempferol content, indicating improved availability. Furthermore, the fermented juice exhibited notable antioxidant activity and suppressed NO production, revealing anti-inflammatory potential. Gene expression analysis confirmed reduced pro-inflammatory markers and elevated anti-inflammatory cytokines. This research highlights the promising potential of fermented kale juice, enriched with Limosilactobacillus strains, as a functional food with combined antioxidant and anti-inflammatory benefits.

Keywords

kale juice; probiotics; Limosilactobacillus reuteri; Limosilactobacillus fermentum; Anti-inflammatory

Subject

Biology and Life Sciences, Food Science and Technology

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