PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Investigation of Fibrin(ogen)lytic, Anticoagulant, and Antithrombotic Effects by Papain, a Natural Cysteine Protease from Carica papaya Latex Using κ-Carrageenan Rat Tail Thrombosis Model
Hyeryeon Yang and Most Nusrat Zahan contributed equally to this work.
Version 1
: Received: 26 August 2023 / Approved: 28 August 2023 / Online: 28 August 2023 (09:48:43 CEST)
How to cite:
Yang, H.; Zahan, M.N.; Yoon, Y.; Kim, K.; Hwang, D.H.; Kim, W.H.; Rho, I.R.; Kim, E.; Kang, C. Investigation of Fibrin(ogen)lytic, Anticoagulant, and Antithrombotic Effects by Papain, a Natural Cysteine Protease from Carica papaya Latex Using κ-Carrageenan Rat Tail Thrombosis Model. Preprints2023, 2023081838. https://doi.org/10.20944/preprints202308.1838.v1
Yang, H.; Zahan, M.N.; Yoon, Y.; Kim, K.; Hwang, D.H.; Kim, W.H.; Rho, I.R.; Kim, E.; Kang, C. Investigation of Fibrin(ogen)lytic, Anticoagulant, and Antithrombotic Effects by Papain, a Natural Cysteine Protease from Carica papaya Latex Using κ-Carrageenan Rat Tail Thrombosis Model. Preprints 2023, 2023081838. https://doi.org/10.20944/preprints202308.1838.v1
Yang, H.; Zahan, M.N.; Yoon, Y.; Kim, K.; Hwang, D.H.; Kim, W.H.; Rho, I.R.; Kim, E.; Kang, C. Investigation of Fibrin(ogen)lytic, Anticoagulant, and Antithrombotic Effects by Papain, a Natural Cysteine Protease from Carica papaya Latex Using κ-Carrageenan Rat Tail Thrombosis Model. Preprints2023, 2023081838. https://doi.org/10.20944/preprints202308.1838.v1
APA Style
Yang, H., Zahan, M.N., Yoon, Y., Kim, K., Hwang, D.H., Kim, W.H., Rho, I.R., Kim, E., & Kang, C. (2023). Investigation of Fibrin(ogen)lytic, Anticoagulant, and Antithrombotic Effects by Papain, a Natural Cysteine Protease from <em>Carica papaya</em> Latex Using κ-Carrageenan Rat Tail Thrombosis Model. Preprints. https://doi.org/10.20944/preprints202308.1838.v1
Chicago/Turabian Style
Yang, H., Euikyung Kim and Changkeun Kang. 2023 "Investigation of Fibrin(ogen)lytic, Anticoagulant, and Antithrombotic Effects by Papain, a Natural Cysteine Protease from <em>Carica papaya</em> Latex Using κ-Carrageenan Rat Tail Thrombosis Model" Preprints. https://doi.org/10.20944/preprints202308.1838.v1
Abstract
While fibrinolytic enzymes and thrombolytic agents offer assistance in treating cardiovascular diseases, the existing options are associated with a range of adverse effects. In our previous research, we successfully identified ficin, a naturally occurring cysteine protease that possesses unique fibrin and fibrinogenolytic enzymes, making it suitable for both preventing and treating cardiovascular disorders linked to thrombosis. Papain is a prominent natural cysteine protease derived from the latex of Carica papaya. The potential role of papain in preventing fibrin(ogen)lytic, anticoagulant, and antithrombotic activities has not yet been investigated. Therefore, we examine how papain influences fibrinogen and the process of blood coagulation. Papain is highly stable at pH 4-11 and 37-60°C by azocasein assay. In addition, SDS gel separation electrophoresis, zymography, and fibrin plate assays were used to determine fibrinogen and fibrinolysis activity. Papain appears to have a molecular weight of around 37 kDa, and is highly effective in degrading fibrin, with a molecular weight of over 75 kDa. Furthermore, papain-based hemostatic performance was confirmed in blood coagulation tests, a blood clot lysis assay, and a κ-carrageenan rat tail thrombosis model, highlighting its strong efficacy in blood coagulation. Papain shows dose-dependent blood clot lysis activity, cleaves fibrinogen chains of Aα, Bβ, and γ-bands and significantly extends prothrombin time (PT) and activated partial thromboplastin time (aPTT). Moreover, the mean length of the infarcted regions in the tails of Sprague-Dawley rats with κ-carrageenan was shorter in rats administered 10 U/kg of papain than in streptokinase-treated rats. Thus, papain, a natural cysteine protease, has distinct fibrin and fibrinogenolytic properties, suggesting its potential for preventing or treating cardiovascular issues and thrombosis-related diseases.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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