Version 1
: Received: 23 August 2023 / Approved: 25 August 2023 / Online: 25 August 2023 (08:33:03 CEST)
How to cite:
Kurevlev, S.; Aghjanayan, A.; Tskhovrebova, L.; Gordon, K.; Polyakov, A.; Ratushnyy, M.; Fatkhudinov, T. DNA Promoter Methylation of the ATM, APC, CDO1, RB1, TP53, WIF1 Genes in Patients with Head and Neck Squamous Cell Carcinoma. Preprints2023, 2023081794. https://doi.org/10.20944/preprints202308.1794.v1
Kurevlev, S.; Aghjanayan, A.; Tskhovrebova, L.; Gordon, K.; Polyakov, A.; Ratushnyy, M.; Fatkhudinov, T. DNA Promoter Methylation of the ATM, APC, CDO1, RB1, TP53, WIF1 Genes in Patients with Head and Neck Squamous Cell Carcinoma. Preprints 2023, 2023081794. https://doi.org/10.20944/preprints202308.1794.v1
Kurevlev, S.; Aghjanayan, A.; Tskhovrebova, L.; Gordon, K.; Polyakov, A.; Ratushnyy, M.; Fatkhudinov, T. DNA Promoter Methylation of the ATM, APC, CDO1, RB1, TP53, WIF1 Genes in Patients with Head and Neck Squamous Cell Carcinoma. Preprints2023, 2023081794. https://doi.org/10.20944/preprints202308.1794.v1
APA Style
Kurevlev, S., Aghjanayan, A., Tskhovrebova, L., Gordon, K., Polyakov, A., Ratushnyy, M., & Fatkhudinov, T. (2023). DNA Promoter Methylation of the ATM, APC, CDO1, RB1, TP53, WIF1 Genes in Patients with Head and Neck Squamous Cell Carcinoma. Preprints. https://doi.org/10.20944/preprints202308.1794.v1
Chicago/Turabian Style
Kurevlev, S., Mikhail Ratushnyy and Timur Fatkhudinov. 2023 "DNA Promoter Methylation of the ATM, APC, CDO1, RB1, TP53, WIF1 Genes in Patients with Head and Neck Squamous Cell Carcinoma" Preprints. https://doi.org/10.20944/preprints202308.1794.v1
Abstract
Head and neck squamous cell carcinoma (HNSCC) takes the sixth place among the most common cancers in the world. Abnormal methylation can be one of the reasons for this cancer. The aim of this study was to investigate the DNA promotor methylation status of cancer-associated genes (ATM, APC, CDO1, RB1, TP53, WIF1) in patients with HNSCC. Bisulfite Conversion and Methylation-Sensitive High-Resolution Melting was used for analysis of the DNA methylation level of normal and tumor tissues in 44 patients. There were significant differences in DNA methylation level between patient’s tumor and normal tissues for CDO1 and WIF1 genes in all subjects and subgroups (p<0.05). In T3 subgroup there was significant correlation between CDO1 gene methylation and age in the normal tissue. The same correlation was detected also for the WIF1 gene methylation in tumor tissue samples in the subgroup with T3 and in normal tissue samples in the subgroup with T4 (p<0,05). In all genes no significant differences were found between the subgroups (T2, T3, T4 stage, primary/recurrent lesion, non-keratinizing/keratinizing SCC, age before/ after 50, smokers/non-smokers) of the patients. Thus, changes in the expression of the CDO1 and WIF1 genes can affect mechanisms of the occurrence and development of HNSCC.
Keywords
DNA methylation; cancer associated genes; head and neck squamous cell carcinoma
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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