Ledwaba, L.K.; Otun, S.; Matsaunyane, L.; Mamputha, S.; Ntushelo, K. Proteomic Profiling of Burkholderia andropogonis. Preprints2023, 2023081695. https://doi.org/10.20944/preprints202308.1695.v1
APA Style
Ledwaba, L.K., Otun, S., Matsaunyane, L., Mamputha, S., & Ntushelo, K. (2023). Proteomic Profiling of Burkholderia andropogonis. Preprints. https://doi.org/10.20944/preprints202308.1695.v1
Chicago/Turabian Style
Ledwaba, L.K., Sipho Mamputha and Khayalethu Ntushelo. 2023 "Proteomic Profiling of Burkholderia andropogonis" Preprints. https://doi.org/10.20944/preprints202308.1695.v1
Abstract
Burkholderia andropogonis is a severe plant pathogen that can infect more than 50 plant species. Although its genome has been sequenced and annotated, various mechanisms underlying its physiology and pathogenicity remain unknown. Some of these mechanisms can be uncovered through understanding its proteome. In this study, sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry analysis were used to profile the proteome of B. andropogonis. Exactly 538 proteins, which belong to 190 protein families performing 88 different functions were identified. The resulting STRING network analysis identified 28 proteins having highly connected interactions with adenosine triphosphate (ATP)-binding-cassette and (ABC) transport system. To the knowledge of the authors, this is the first comprehensive proteomic study on B. andropogonis.
Keywords
Burkholderia andropogonis; mass spectrometry; sodium dodecyl polyacrylamide gel electrophoresis; proteomic profile
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
