Version 1
: Received: 19 August 2023 / Approved: 21 August 2023 / Online: 22 August 2023 (07:44:09 CEST)
How to cite:
Mot, A.C.; Botis, A.C.; Silaghi-Dumitrescu, R. A Polyethylene Glycol Derivatized Laccase with Improved Thermal Resistance. Preprints2023, 2023081498. https://doi.org/10.20944/preprints202308.1498.v1
Mot, A.C.; Botis, A.C.; Silaghi-Dumitrescu, R. A Polyethylene Glycol Derivatized Laccase with Improved Thermal Resistance. Preprints 2023, 2023081498. https://doi.org/10.20944/preprints202308.1498.v1
Mot, A.C.; Botis, A.C.; Silaghi-Dumitrescu, R. A Polyethylene Glycol Derivatized Laccase with Improved Thermal Resistance. Preprints2023, 2023081498. https://doi.org/10.20944/preprints202308.1498.v1
APA Style
Mot, A.C., Botis, A.C., & Silaghi-Dumitrescu, R. (2023). A Polyethylene Glycol Derivatized Laccase with Improved Thermal Resistance. Preprints. https://doi.org/10.20944/preprints202308.1498.v1
Chicago/Turabian Style
Mot, A.C., Antoanela C Botis and Radu Silaghi-Dumitrescu. 2023 "A Polyethylene Glycol Derivatized Laccase with Improved Thermal Resistance" Preprints. https://doi.org/10.20944/preprints202308.1498.v1
Abstract
Laccases are versatile and relatively accessible catalysts with practical/industrial applications. Their proteic nature entails limited stability under non-native conditions, especially such as those that would be required for industrial applications. Here, derivatization of a laccase from Sclerotinia sclerotiorum is reported, using an (methyl-PEG12)3-PEG4) N-succinimide ester in order to improve the thermostability of the enzyme. The PEGylated laccase is characterized using gel electrophoresis, size-exclusion chromatography, catalytic parameters and thermal stability. A 50% increase in residual activity after incubation at 50°C for up to two hours was achieved for the PEGylated laccase compared to the native enzyme.
Keywords
laccase; PEG; thermostability
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
