Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Influence of Reaction Time on Silver Nanoparticles-Catalyzed Oxidation of 3,3’,5,5’-Tetramethylbenzidine

Version 1 : Received: 16 August 2023 / Approved: 16 August 2023 / Online: 17 August 2023 (09:57:28 CEST)

How to cite: Hormozi Jangi, S.R. Influence of Reaction Time on Silver Nanoparticles-Catalyzed Oxidation of 3,3’,5,5’-Tetramethylbenzidine. Preprints 2023, 2023081273. https://doi.org/10.20944/preprints202308.1273.v1 Hormozi Jangi, S.R. Influence of Reaction Time on Silver Nanoparticles-Catalyzed Oxidation of 3,3’,5,5’-Tetramethylbenzidine. Preprints 2023, 2023081273. https://doi.org/10.20944/preprints202308.1273.v1

Abstract

Influence of reaction time on silver nanoparticles-catalyzed oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) was evaluated via probing the oxidation process by recording the UV-Visible absorbance of the resulting colored products of TMB oxidation by hydrogen peroxide in the presence of silver nanoparticles as peroxides mimics. The time curve of the oxidation process was constructed, revealed that the concentration of the oxidation product was increased by increasing the reaction time and then levled off because the nanozyme active nodes were saturated after a certain reaction time and consequently the steady-state condition can be observed. The maximal activity of the silver nanozymes was achieved after a very short reaction time as short as 3.0 min and after this time, the color intensity of the oxidation products remained constant, revealing saturation of active nodes of nanoparticles with nanozyme-substrate.

Keywords

Silver nanoparticles; peroxidase mimics; time influence on oxidation; nanozyme active nodes

Subject

Chemistry and Materials Science, Analytical Chemistry

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