Version 1
: Received: 14 August 2023 / Approved: 15 August 2023 / Online: 16 August 2023 (20:32:54 CEST)
How to cite:
Alenazi, N.A. Antibacterial and Anti-quorum Sensing Activity of PAMAM G4 Loaded Quorum Quencher Peptide against MRSA, Staphylococcus aureus and Agr Mutants. Preprints2023, 2023081201. https://doi.org/10.20944/preprints202308.1201.v1
Alenazi, N.A. Antibacterial and Anti-quorum Sensing Activity of PAMAM G4 Loaded Quorum Quencher Peptide against MRSA, Staphylococcus aureus and Agr Mutants. Preprints 2023, 2023081201. https://doi.org/10.20944/preprints202308.1201.v1
Alenazi, N.A. Antibacterial and Anti-quorum Sensing Activity of PAMAM G4 Loaded Quorum Quencher Peptide against MRSA, Staphylococcus aureus and Agr Mutants. Preprints2023, 2023081201. https://doi.org/10.20944/preprints202308.1201.v1
APA Style
Alenazi, N.A. (2023). Antibacterial and Anti-quorum Sensing Activity of PAMAM G4 Loaded Quorum Quencher Peptide against MRSA, Staphylococcus aureus and Agr Mutants. Preprints. https://doi.org/10.20944/preprints202308.1201.v1
Chicago/Turabian Style
Alenazi, N.A. 2023 "Antibacterial and Anti-quorum Sensing Activity of PAMAM G4 Loaded Quorum Quencher Peptide against MRSA, Staphylococcus aureus and Agr Mutants" Preprints. https://doi.org/10.20944/preprints202308.1201.v1
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) exerts a considerable concern in healthcare and the community. Globally MRSA strains cause numerous infections with high morbidity and mortality rates. The success of MRSA is a result of the extensive production of virulence factors. With the limited treatment options for the MRSA infection and growing resistance problem, an urgent call is needed to search for an alternative to antibiotics. One of the promising strategies is targeting the quorum sensing (QS) system, of the MRSA, by using a dendrimer loaded with kinase inhibitor peptide. This study formulates a poly-amidoamine dendrimers (PAMAM) G4 dendrimers loaded with Quorum Quencher (QQ), QQ3 peptide which is a Histidine Kinase inhibitor. The physicochemical properties of the formula were characterized using different in-vitro analysis method such as Zetasizer and transmission electron microscope (TEM). The antibacterial activity and anti-virulence effect of the formula was tested against MRSA and different mutant stains using growth assay, haemolysis assay and biofilm assay. Overall, QQ3-G4 complex showed antibacterial and anti-virulence activity and it can be a potential alternative treatment to overcome antimicrobial resistance and treat MRSA infection.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
