Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Morphological Reconstruction of a Critical Size Bone Defect in the Maxillofacial Region Using Modified Chitosan in Rats with sub-Compensated Type I Diabetes Mellitus

Igor N Bolshakov
* ORCID logo ,
Nadezhda N. Patlataya
,
Vladimir A. Khorzhevskii
,
Anatoli A. Levenets
,
Nadezhda N Medvedeva
,
Mariya A Cherkashina
,
Matvey M. Nikolaenko
,
Ekaterina I Ryaboshapko
,
Anna E. Dmitrienko
Version 1 : Received: 14 August 2023 / Approved: 15 August 2023 / Online: 16 August 2023 (05:40:23 CEST)

A peer-reviewed article of this Preprint also exists.

Patlataya, N.N.; Bolshakov, I.N.; Khorzhevskii, V.A.; Levenets, A.A.; Medvedeva, N.N.; Cherkashina, M.A.; Nikolaenko, M.M.; Ryaboshapko, E.I.; Dmitrienko, A.E. Morphological Reconstruction of a Critical-Sized Bone Defect in the Maxillofacial Region Using Modified Chitosan in Rats with Sub-Compensated Type I Diabetes Mellitus. Polymers 2023, 15, 4337. Patlataya, N.N.; Bolshakov, I.N.; Khorzhevskii, V.A.; Levenets, A.A.; Medvedeva, N.N.; Cherkashina, M.A.; Nikolaenko, M.M.; Ryaboshapko, E.I.; Dmitrienko, A.E. Morphological Reconstruction of a Critical-Sized Bone Defect in the Maxillofacial Region Using Modified Chitosan in Rats with Sub-Compensated Type I Diabetes Mellitus. Polymers 2023, 15, 4337.

Abstract

It is known that complexes based on natural polysaccharides are able to eliminate bone defects. Prolonged hyperglycemia means low bone regeneration and a chronic inflammatory response. The purpose of the study was to increase the efficiency of early bone formation in a cavity of critical size in diabetes mellitus in the experiment. The polyelectrolyte complex contains high molecular ascorbate of chitosan, chondroitin sulfate, sodium hyaluronate, heparin, adgelon serum growth factor, sodium alginate and amorphous nanohydroxyapatite (CH-SA-HA). Studies were conducted on 5 groups of white female Wistar rats: group 1 - regeneration of a bone defect in healthy animals under a blood clot; group 2 - regeneration of a bone defect under a blood clot in animals with diabetes mellitus; group 3 - bone regeneration in animals with diabetes mellitus after filling the bone cavity with a collagen sponge; group 4 - filling of a bone defect with a CH-SA-HA construct in healthy animals; Group 5 - filling of a bone defect CH-SA-HA in animals with diabetes mellitus. Implantation of the CH-SA-HA construct into bone cavities in type I diabetic rats can accelerate the rate of bone tissue repair. The inclusion of modifying polysaccharides and apatite agents in the construction may be a prospect for further improvement of the properties of implants.

Keywords

modified chitosan; polyelectrolyte complex CH-SA-HA; rats; type I diabetes mellitus; bone critical size cavity; morphological reconstruction; histomorphometric criteria

Subject

Medicine and Pharmacology, Dentistry and Oral Surgery

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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