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Natural Biocontrol of Athelia rolfsii isolate INVEPAR-05 in Stevia rebaudiana Bertoni: Exploring the Biocontrol Potential of Native Trichoderma spp. Strains
Rodriguez-Paez, L.A.; Jaraba-Navas, J.D.D.; Pineda-Rodriguez, Y.Y.; Begambre-Hernandez, M.; Pompelli, M.F.; Jimenez-Ramirez, A.M.; Gil-Rocha, A.; Jarma-Orozco, A.; Combatt-Caballero, E.; Aviña-Padilla, K.; Jamal, A.; Oloriz-Ortega, M.I.; Rodríguez, N.V. Natural Biocontrol of Athelia rolfsii isolate INVEPAR-05 in Stevia rebaudiana Bertoni: Exploring the Biocontrol Potential of Native Trichoderma spp. Strains. Preprints2023, 2023081062. https://doi.org/10.20944/preprints202308.1062.v1
APA Style
Rodriguez-Paez, L.A., Jaraba-Navas, J.D.D., Pineda-Rodriguez, Y.Y., Begambre-Hernandez, M., Pompelli, M.F., Jimenez-Ramirez, A.M., Gil-Rocha, A., Jarma-Orozco, A., Combatt-Caballero, E., Aviña-Padilla, K., Jamal, A., Oloriz-Ortega, M.I., & Rodríguez, N.V. (2023). Natural Biocontrol of <em>Athelia rolfsii</em> isolate INVEPAR-05 in <em>Stevia rebaudiana</em> Bertoni: Exploring the Biocontrol Potential of Native <em>Trichoderma </em>spp. Strains. Preprints. https://doi.org/10.20944/preprints202308.1062.v1
Chicago/Turabian Style
Rodriguez-Paez, L.A., Maria Ileana Oloriz-Ortega and Novisel Veitía Rodríguez. 2023 "Natural Biocontrol of <em>Athelia rolfsii</em> isolate INVEPAR-05 in <em>Stevia rebaudiana</em> Bertoni: Exploring the Biocontrol Potential of Native <em>Trichoderma </em>spp. Strains" Preprints. https://doi.org/10.20944/preprints202308.1062.v1
Abstract
Stevia rebaudiana (Bert.) is a natural sweetener with diterpenic glycosides, offering 300 times more sweetness than sucrose, and boasting low-calorie content, along with medicinal and therapeutic properties. To address a limited genetic diversity, a germplasm bank comprising 115 segregated lines of stevia was established in Colombia. Notwithstanding, the fungus Athelia rolfsii adversely impacted key stevia lines, leading to wilting and plant mortality. For this reason our study aimed to explore the biocontrol potential of native strains of Trichoderma spp., isolated from productive lots in Colombia's Cordoba department, as a solution to manage A. rolfsii. Soil samples underwent serial dilution technique (103 conidia.mL-1) to obtain Trichoderma spp. isolates, which were thoroughly morphologically and molecularly characterized. A total five native strains were identified through sequencing the PCR products using universal ITS primers, and comparisons with the GENBANK database confirmed their identity (OK310695.1, ON237703.1, ON238106.1, ON237737.1, and ON237923.1). Their biocontrol efficacy against A. rolfsii was evaluated using the dual culture technique. Remarkably, all strains exhibited significant biocontrol capacity against the A. rolfsii isolate INVEPAR-05 (OK271308.1), except for Trichoderma viride INVEPAR-T10 (ON237923.1). These findings suggest that Trichoderma spp. could be a potential option to manage the fungus in stevia.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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