Version 1
: Received: 12 August 2023 / Approved: 14 August 2023 / Online: 14 August 2023 (11:19:30 CEST)
Version 2
: Received: 5 October 2023 / Approved: 6 October 2023 / Online: 6 October 2023 (08:32:17 CEST)
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers2023, 15, 4232.
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers 2023, 15, 4232.
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers2023, 15, 4232.
Patlataya, N.N.; Bolshakov, I.N.; Levenets, A.A.; Medvedeva, N.N.; Khorzhevskii, V.A.; Cherkashina, M.A. Experimental Early Stimulation of Bone Tissue Neo-Formation for Critical Size Elimination Defects in the Maxillofacial Region. Polymers 2023, 15, 4232.
Abstract
Abstract: A biomaterial is proposed for closing extensive bone defects in the maxillofacial region. The composition of the biomaterial includes high-molecular chitosan, chondroitin sulfate, hyaluronate, heparin, alginate and inorganic nanostructured hydroxyapatite. The purpose of the study is to demonstrate morphological and histological early signs of reconstruction of a bone cavity of critical size. The studies were carried out on 84 white female rats weighing 200-250 g. The study group consisted of 84 subjects in total, 40 in the experimental group and 44 in the control group. In all animals, three-walled bone defects measuring 0.5 x 04 x 05 cm were applied subperiosteally in the region of the angle of the lower jaw and filled in experimental group using lyophilized gel mass of chitosan-alginate-hydroxyapatite (CH-SA-HA). In control animals, the bone cavities were filled with an auto-blood clot after bone trepanation and bleeding. The observation periods are 3.5.7 days, 2.3.4.6.8 and 10 weeks. The control of bone regeneration was carried out using multiple morphological and histological analyses. Results showed that following implantation the chitosan construct actively replaced early-stage defects with the formation of a full-fledged new bone tissue as compared to the control group. Already, by the 7th day morphological analysis showed that formation of spongy bone tissue could be seen. After 2 weeks there was a pronounced increase in bone volume (P<0.01), and at 6 weeks after surgical intervention the closure of the defect was 70-80%, after 8 weeks - 100% without violation of bone morphology with a high degree of mineralization. Thus, the use of modified chitosan after filling eliminates bone defects of a critical size in the maxillofacial region, reveals early signs of bone regeneration, and serves as a promising material in reconstructive dentistry.
Keywords
experimental critical size bone defect; maxillofacial area; chitosan; bone formation; morphometry; rats
Subject
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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