Version 1
: Received: 10 August 2023 / Approved: 11 August 2023 / Online: 11 August 2023 (10:16:57 CEST)
How to cite:
Hormozi Jangi, S.R. Investigating the Biochemical Stability and Kinetic Performances of SiO2@AuNPs Nanocomposite as High Throughput Peroxidase Alternatives. Preprints2023, 2023080921. https://doi.org/10.20944/preprints202308.0921.v1
Hormozi Jangi, S.R. Investigating the Biochemical Stability and Kinetic Performances of SiO2@AuNPs Nanocomposite as High Throughput Peroxidase Alternatives. Preprints 2023, 2023080921. https://doi.org/10.20944/preprints202308.0921.v1
Hormozi Jangi, S.R. Investigating the Biochemical Stability and Kinetic Performances of SiO2@AuNPs Nanocomposite as High Throughput Peroxidase Alternatives. Preprints2023, 2023080921. https://doi.org/10.20944/preprints202308.0921.v1
APA Style
Hormozi Jangi, S.R. (2023). Investigating the Biochemical Stability and Kinetic Performances of SiO2@AuNPs Nanocomposite as High Throughput Peroxidase Alternatives. Preprints. https://doi.org/10.20944/preprints202308.0921.v1
Chicago/Turabian Style
Hormozi Jangi, S.R. 2023 "Investigating the Biochemical Stability and Kinetic Performances of SiO2@AuNPs Nanocomposite as High Throughput Peroxidase Alternatives" Preprints. https://doi.org/10.20944/preprints202308.0921.v1
Abstract
In this study, considering the peroxidase-like activity of the SiO2@AuNPs nanocomposite, their biochemical properties including, pH stability, cycling stability, shelf stability, and kinetic parameters were investigated. The results revealed that the as-mentioned SiO2@AuNPs nanocomposite reveal its maximal activity at pH=4.0 along with saving 83.3% of its maximal activity at pH=5.0. Besides, the reusability and shelf-storage studies exhibited that the SiO2@AuNPs nanocomposite retained 90% and 100% of its initial activity after 5 operational cycles and 30 days of storage, in order. The kinetic parameters of the as-prepared nanozymes were calculated using Menten kinetic model, revealing a Vmax of 1.35 µM min-1 and a Km as low as 0.06 mM for the SiO2@AuNPs nanocomposite. Based on the results of this work, the as-prepared SiO2@AuNPs nanocomposite with intrinsic peroxidase-like activity shows high substrate affinity and catalytic efficiency along with excellent cycling- and shelf-stability, making them suitable for application in peroxidase-mediated reactions instead of the native enzyme.
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
