Wang, Q.; Xu, Z.; Yang, X.; Zhao, R.; Li, X.; Li, S.; Li, X.; Zeng, J. Identification and validation of blood miRNAs as diagnostic markers of gastric cancer. Preprints2023, 2023080543. https://doi.org/10.20944/preprints202308.0543.v1
APA Style
Wang, Q., Xu, Z., Yang, X., Zhao, R., Li, X., Li, S., Li, X., & Zeng, J. (2023). Identification and validation of blood miRNAs as diagnostic markers of gastric cancer. Preprints. https://doi.org/10.20944/preprints202308.0543.v1
Chicago/Turabian Style
Wang, Q., Xize Li and Jiping Zeng. 2023 "Identification and validation of blood miRNAs as diagnostic markers of gastric cancer" Preprints. https://doi.org/10.20944/preprints202308.0543.v1
Abstract
In order to provide a new direction for the diagnosis and treatment of gastric cancer, the differentially expressed blood-based miRNAs in gastric carcinoma were obtained and analyzed from the datasets GSE113486, GSE112264 and GSE113740 by the SangerBox, the miRNA - target genes prediction, GO and KEGG functional enrichment analyses with DAVID 6.8, and the STRING database with Cytoscape, respectively. The correlation between the key miRNAs expression and the prognosis and diagnostic value of gastric cancer patients were detected by survival prognosis and ROC curve. QRT-PCR was used to detect the possible key miRNAs expression in human blood species. The dataset GSE108307 which shows the miRNAs affected by H. pylori infection was analyzed by GEO2R. The expression level of CagA and the clinically used gastric cancer markers were detected in human blood species for contrast. Five key blood-based miRNAs were determined, including hsa-miR-124-3p, hsa-miR-125a-3p, hsa-miR-29b-3p, hsa-miR-4276, and hsa-miR-575.The detection in human blood species as well as the cross analysis with H. pylori infection and clinically used markers expression suggested the importance and efficacy of the specific miRNAs in the early diagnosis and monitoring of gastric carcinoma. The study identified potential novel blood miRNA biomarkers of gastric cancer by bioinformatics and human blood species detection, which may give a new possibility of the important biomarkers for the diagnosis and prognosis of gastric cancer.
Medicine and Pharmacology, Oncology and Oncogenics
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