Zalejski, J.; Sun, J.; Sharma, A. Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging. J. Imaging2023, 9, 192.
Zalejski, J.; Sun, J.; Sharma, A. Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging. J. Imaging 2023, 9, 192.
Zalejski, J.; Sun, J.; Sharma, A. Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging. J. Imaging2023, 9, 192.
Zalejski, J.; Sun, J.; Sharma, A. Unravelling the Mystery inside Cells by Using Single-Molecule Fluorescence Imaging. J. Imaging 2023, 9, 192.
Abstract
Live cell imaging is a powerful technique to study the dynamics and mechanics of various biological molecules like proteins, organelles, DNA, and RNA. With the rapid evolution of optical microscopy, our understanding of how these molecules is implicated in the cells’ most critical physiological roles deepens. Here in this review, we focus on how spatiotemporal nanoscale live cell imaging on a single molecule level allows for profound contributions towards new discoveries in life science. This review will start by summarizing how single molecule tracking has been used to analyze membrane dynamics, receptor ligand interactions, protein-protein interactions, inner- and extracellular transport, gene expression/transcription, and whole organelle tracking. We then move on to how current authors are trying to improve single molecule tracking and overcome current limitations by offering new ways in labeling protein of interests, multi-channel/color detection, improvements in time laps imaging, and new methods and programs to analyze colocalization and movement of targets. We later discuss how single molecule tracking can be a beneficial tool used for medical diagnosis. Finally wrapping up with limitation and future perspective of single molecule tracking and total internal refection microscopy.
Keywords
Protein-Protein Interaction; Medical Diagnostic; Cell Imaging and Signaling; Total Internal Reflection Fluorescence; Protein dynamics.
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
