Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

First Report of Hemp Fusarium wilt Caused by Fusarium oxysporum in Croatia

Tomislav Duvnjak
* ORCID logo ,
Karolina Vrandecic
,
Aleksandra Sudarić
,
Jasenka Cosic
,
Tamara Siber
ORCID logo ,
Maja Matosa Kocar
ORCID logo
Version 1 : Received: 4 August 2023 / Approved: 4 August 2023 / Online: 4 August 2023 (13:54:45 CEST)

A peer-reviewed article of this Preprint also exists.

Duvnjak, T.; Vrandecic, K.; Sudaric, A.; Cosic, J.; Siber, T.; Matosa Kocar, M. First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia. Plants 2023, 12, 3305. Duvnjak, T.; Vrandecic, K.; Sudaric, A.; Cosic, J.; Siber, T.; Matosa Kocar, M. First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia. Plants 2023, 12, 3305.

Abstract

Wilted hemp (Cannabis sativa L.) plants were observed in August 2019 in commercial fields around Osijek, Croatia. Plants and roots with disease symptoms were collected. The single-spored isolates produced septate cottony white to light pink aerial mycelium and purple undersurface on potato dextrose agar (PDA). Smooth and hyaline hyphae were branched and septate. Macroconidia were fusiform to sickle-shaped with foot-shaped basal cell, elongated apical cell and three to five septa. Sequencing of the internal transcribed spacer and the partial elongation factor 1-α gene identified species as Fusarium oxysporum. Artificial infection fulfills Koch’s postulates, producing plants which showed stunted growth and wilt symptoms similar to those observed in the commercial fields. Control seedlings remained symptomless and healthy. To the best of our knowledge, this is the first report of hemp Fusarium wilt causing F. oxysporum in Croatia. Considering that F. oxysporum has been reported in main field crops in Croatia, the presence of this pathogen could cause economically significant hemp production decreases, especially in humid and cold springs and susceptible varieties.

Keywords

hemp; Fusarium oxysporum; wilt; Croatia

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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