Maeda, N.; Yumoto, T.; Xiong, G.; Hasegawa, Y. Characterization and Stability of a Novel Toxin in Scallop Mantle Tissue. Foods2023, 12, 3224.
Maeda, N.; Yumoto, T.; Xiong, G.; Hasegawa, Y. Characterization and Stability of a Novel Toxin in Scallop Mantle Tissue. Foods 2023, 12, 3224.
Maeda, N.; Yumoto, T.; Xiong, G.; Hasegawa, Y. Characterization and Stability of a Novel Toxin in Scallop Mantle Tissue. Foods2023, 12, 3224.
Maeda, N.; Yumoto, T.; Xiong, G.; Hasegawa, Y. Characterization and Stability of a Novel Toxin in Scallop Mantle Tissue. Foods 2023, 12, 3224.
Abstract
We previously showed that feeding mice a diet containing 1% mantle tissue decreased food consumption, leading to death. We also isolated and identified toxic substances in the mantle tissue. In the present study, we investigated the characteristics and stability of mantle tissue toxicity. Treatment of mantle tissue with 1 mM HCl, 1 mM NaOH, 1 mM dithiothreitol, and 1 mM H2O2 and heating did not reduce the toxicity of mantle tissue in mice. These results suggest that mantle toxins are stable in tissues, particularly when exposed to acidic and digestive enzymes. We examined whether mantle tissue exhibited acute toxicity. Diets containing 1% and 20% mantle extract showed similar levels of toxicity, demonstrating that feeding of mantle tissue does not lead to acute toxicity. Finally, we examined the toxicity of the mantle tissue against small intestinal tissue. Chronic feeding of mantle tissue to mice changed the color of the small intestine. Real-time PCR analysis showed that mantle tissue feeding caused changes of inflammation and endoplasmic reticulum stress markers in the small intestine. These results suggest that feeding of mantle tissue causes toxicity after causing initial damage to the small intestinal tissue.
Keywords
mantle tissue; novel toxin; scallop; small intestine
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
