Version 1
: Received: 18 July 2023 / Approved: 19 July 2023 / Online: 19 July 2023 (13:51:54 CEST)
Version 2
: Received: 31 August 2023 / Approved: 31 August 2023 / Online: 31 August 2023 (13:17:58 CEST)
How to cite:
Scott, H.C.; Draganov, S.D.; Yu, Z.; Kessler, B.M.; Pinto-Fernández, A. Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia. Preprints2023, 2023071356. https://doi.org/10.20944/preprints202307.1356.v2
Scott, H.C.; Draganov, S.D.; Yu, Z.; Kessler, B.M.; Pinto-Fernández, A. Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia. Preprints 2023, 2023071356. https://doi.org/10.20944/preprints202307.1356.v2
Scott, H.C.; Draganov, S.D.; Yu, Z.; Kessler, B.M.; Pinto-Fernández, A. Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia. Preprints2023, 2023071356. https://doi.org/10.20944/preprints202307.1356.v2
APA Style
Scott, H.C., Draganov, S.D., Yu, Z., Kessler, B.M., & Pinto-Fernández, A. (2023). Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia. Preprints. https://doi.org/10.20944/preprints202307.1356.v2
Chicago/Turabian Style
Scott, H.C., Benedikt M. Kessler and Adán Pinto-Fernández. 2023 "Targeted Mass Spectrometry Reveals Interferon-Dependent Eicosanoid and Fatty Acid Alterations in Chronic Myeloid Leukaemia" Preprints. https://doi.org/10.20944/preprints202307.1356.v2
Abstract
Bioactive lipids are involved in cellular signalling events with links to human disease. Many of these are involved in inflammation under normal and pathological conditions. Despite being attractive molecules from a pharmacological point of view, detection and quantification of lipids has been a major challenge. Here, we have optimised a liquid chromatography dynamic multiple reaction monitoring targeted mass spectrometry (LC-dMRM-MS) approach to profile eicosanoids and fatty acids in biological samples. In particular, by applying this analytic workflow to study a cellular model of Chronic Myeloid Leukaemia (CML), we found that intra- and extra-cellular 2-Arachidonoylglycerol (2-AG), intracellular Arachidonic Acid (AA), and extracellular Prostaglandin F2α (PGF2α), 5-Hydroxyeicosatetraenoic acid (5-HETE), Palmitic acid (PA, C16:0) and Stearic acid (SA, C18:0) were altered in response to immunomodulation by type I Interferon (IFN-I), a currently approved treatment for CML. Our observations indicate changes in eicosanoid and fatty acid metabolism with potential relevance in the context of cancer inflammation and CML.
Keywords
Bioactive Lipids; Eicosanoids; Fatty Acids; Mass Spectrometry; Lipidomics; Innate Immunity; Type-I Interferon Response; Chronic Myeloid Leukaemia; Cancer Inflammation; Cancer Metabolism
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter: Adan Pinto-Fernandez
Commenter's Conflict of Interests: Author