Version 1
: Received: 18 July 2023 / Approved: 18 July 2023 / Online: 19 July 2023 (09:11:19 CEST)
How to cite:
Tuyet, N.L.T.; Kim, J. Acinetobacter thutiue sp. nov. and Acinetobacter kontum sp. nov., Isolated from Motorbike Repairing Workshop Soil. Preprints2023, 2023071271. https://doi.org/10.20944/preprints202307.1271.v1
Tuyet, N.L.T.; Kim, J. Acinetobacter thutiue sp. nov. and Acinetobacter kontum sp. nov., Isolated from Motorbike Repairing Workshop Soil. Preprints 2023, 2023071271. https://doi.org/10.20944/preprints202307.1271.v1
Tuyet, N.L.T.; Kim, J. Acinetobacter thutiue sp. nov. and Acinetobacter kontum sp. nov., Isolated from Motorbike Repairing Workshop Soil. Preprints2023, 2023071271. https://doi.org/10.20944/preprints202307.1271.v1
APA Style
Tuyet, N.L.T., & Kim, J. (2023). <em>Acinetobacter thutiue</em> sp. nov. and <em>Acinetobacter kontum</em> sp. nov., Isolated from Motorbike Repairing Workshop Soil. Preprints. https://doi.org/10.20944/preprints202307.1271.v1
Chicago/Turabian Style
Tuyet, N.L.T. and Jaisoo Kim. 2023 "<em>Acinetobacter thutiue</em> sp. nov. and <em>Acinetobacter kontum</em> sp. nov., Isolated from Motorbike Repairing Workshop Soil" Preprints. https://doi.org/10.20944/preprints202307.1271.v1
Abstract
Two isolates of Acinetobacter were isolated from the soil samples of motorbike repairing workshop floors in Vietnam, where motorcycle/scooter repairs generate various types of hazardous waste, including mainly used waste oil and fluids. Two isolates were Gram-stain-negative, coccobacilli-shaped, aerobic, non-motile, non-hemolytic, oxidase-negative, and catalase-positive, designated strains VNH17T and VNK23T. Growth of strains was inhibited by NaCl concentrations above 3% and grew at an optimal temperature of 20-35°C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that two isolated strains formed a lineage within the family Moraxellaceae and clustered as members of the genus Acinetobacter. The predominant respiratory quinones of VNH17T and VNK23T are ubiquinone 9 and ubiquinone 8, and the polar lipids are mainly composed of diphosphatidyl glycerol, phosphatidylglycerol, and phosphatidylethanolamine. The major cellular fatty acids are C18:1ω9c, C16:0 and summed feature 3 (C16:1ω7c and/or C16 : 1ω6c). The DNA G+C content of VNH17T and VNK23T was 42.07% and 41.75%, respectively. In addition, the average nucleotide identity and in silico DNA-DNA hybridization relatedness values between VNH17T and VNK23T as well as between they and phylogenetically related type strains were below the threshold values used for species delineation. Based on genomic, chemotaxonomic, phenotypic and phylogenomic analyses, the isolated strains represent novel species in the genus Acinetobacter, for which the names Acinetobacter thutiue sp. nov. (type strain VNH17T = KACC 23003 = CCTCC AB 2023063), Acinetobacter kontum sp. nov. (type strain VNK23T = KACC 23002 = CCTCC AB 2023064) are proposed.
Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
