Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Control of the Growth of Listeria monocytogenes in Cooked Ham through Combinations of Natural Ingredients

Version 1 : Received: 17 July 2023 / Approved: 18 July 2023 / Online: 18 July 2023 (07:31:24 CEST)

A peer-reviewed article of this Preprint also exists.

Nieto, G.; Peñalver, R.; Ortuño, C.; Hernández, J.D.; Guillén, I. Control of the Growth of Listeria Monocytogenes in Cooked Ham through Combinations of Natural Ingredients. Foods 2023, 12, 3416, doi:10.3390/foods12183416. Nieto, G.; Peñalver, R.; Ortuño, C.; Hernández, J.D.; Guillén, I. Control of the Growth of Listeria Monocytogenes in Cooked Ham through Combinations of Natural Ingredients. Foods 2023, 12, 3416, doi:10.3390/foods12183416.

Abstract

In the food industry, Listeria is a standard control microorganism in ready-to-eat food since it may be pathogenic and cause a disease called listeriosis. The objective of the present study was to carry out a challenge test to verify the efficiency of different combinations of natural antimicrobial ingredients, against Listeria monocytogenes, to be used in ready-to-eat foods. Six different formulations of cooked ham were elaborated: a control, and five different formulations. An initial inoculation of 2 log cycles was used in the different products and the growth of Listeria was monitored at different temperatures and times (4° C for 17 w; 7ºC for 12 w). Control samples showed a progressive growth, reaching 5-6 log after 3 or 4 weeks. The rest of samples showed constant counts of Listeria during the all study. Only samples containing: 100 ppm nitrite + 250 PPM ascorbic acid+0,7% PRS DV-5 did not control the growth of Listeria at 7 ° C after 7 w of storage. The results obtained allowed to classified the cooked ham elaborated using any natural ingredients combinations as a “Ready-to-eat food unable to support the growth of L. monocytogenes other than those intended for infants and for special medical purposes”.

Keywords

Challenge test; Listeria monocytogenes; natural ingredients combinations, food safety.

Subject

Biology and Life Sciences, Food Science and Technology

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