Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

p53R248Q Induces Invasion and Migration Through Overexpression of miR-182-5p in Cancer

Tzitzijanik Madrigal
ORCID logo ,
Daniel Ortega-Bernal
,
Luis A Herrera
,
Claudia Haydée González De La Rosa
,
Guadalupe Domínguez-Gómez
,
Elena Aréchaga-Ocampo
,
José Díaz-Chávez
* ORCID logo
Version 1 : Received: 11 July 2023 / Approved: 12 July 2023 / Online: 12 July 2023 (12:10:37 CEST)

A peer-reviewed article of this Preprint also exists.

Madrigal, T.; Ortega-Bernal, D.; Herrera, L.A.; González-De la Rosa, C.H.; Domínguez-Gómez, G.; Aréchaga-Ocampo, E.; Díaz-Chávez, J. Mutant p53 Gain-of-Function Induces Migration and Invasion through Overexpression of miR-182-5p in Cancer Cells. Cells 2023, 12, 2506. Madrigal, T.; Ortega-Bernal, D.; Herrera, L.A.; González-De la Rosa, C.H.; Domínguez-Gómez, G.; Aréchaga-Ocampo, E.; Díaz-Chávez, J. Mutant p53 Gain-of-Function Induces Migration and Invasion through Overexpression of miR-182-5p in Cancer Cells. Cells 2023, 12, 2506.

Abstract

Some p53 mutants lose their tumor suppressor activity and acquire new oncogenic functions, known as a gain of function. Recent studies have shown that p53 mutants can exert oncogenic effects through specific miRNAs. We identified the differentially expressed miRNA profiles of the three most frequent p53 mutants (p53R273C, p53R248Q, and p53R175H) in the Saos-2 cell line (null p53) transfected as compared with p53WT transfected cells. The association between these miRNAs and the signaling pathways in which they might participate was performed through the miRPath Software. QRT-PCR was employed to validate the miRNAs profiles. In addition, we explored if the induction of cell migration and invasion by the p53R48Q mutant was dependent on the miR-182-5p. We observed that p53 mutants have an overall negative effect on miRNA expression. Likewise, we found miRNAs differentially expressed associated with regulating Oncogenic signaling pathways. We found overexpression of miR-182-5p, which is associated with processes such as cell migration and invasion. Inhibition of mutant p53R248Q and miR-182-5p increased FOXF2-MTSS1 levels and decreased cell migration and invasion. Our results suggest that p53 mutants increase the expression of miR-182-5p, and this miRNA is also necessary to induce cell migration and invasion by the p53R248Q mutant in a cancer cell model.

Keywords

miRNAs; Mutant p53; Gain of function; Cancer

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.