Version 1
: Received: 7 July 2023 / Approved: 11 July 2023 / Online: 12 July 2023 (09:20:30 CEST)
How to cite:
Nworji, O.; Ogana, J.; Orji, E.; ohanenye, I.; Ezeigwe, O.; Enemor, A. The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis. Preprints2023, 2023070778. https://doi.org/10.20944/preprints202307.0778.v1
Nworji, O.; Ogana, J.; Orji, E.; ohanenye, I.; Ezeigwe, O.; Enemor, A. The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis. Preprints 2023, 2023070778. https://doi.org/10.20944/preprints202307.0778.v1
Nworji, O.; Ogana, J.; Orji, E.; ohanenye, I.; Ezeigwe, O.; Enemor, A. The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis. Preprints2023, 2023070778. https://doi.org/10.20944/preprints202307.0778.v1
APA Style
Nworji, O., Ogana, J., Orji, E., ohanenye, I., Ezeigwe, O., & Enemor, A. (2023). The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis. Preprints. https://doi.org/10.20944/preprints202307.0778.v1
Chicago/Turabian Style
Nworji, O., Obiajulu Ezeigwe and Azubuike Enemor. 2023 "The Chloroplast Genome of a V. amygdalina Specie Isolated from Awka, Nigeria: Comparative and Phylogenetic Analysis" Preprints. https://doi.org/10.20944/preprints202307.0778.v1
Abstract
Vernonia amygdalina is an important nutritional and medicinal plant that is used to treat various diseases such as malaria and diabetes in Nigeria. In this study, we sequenced, assembled, and annotated the complete nucleotide sequence of the chloroplast genome of a V. amygdalina species isolated in Awka, Nigeria. The chloroplast genome is 153 149 bp in length and has a conserved structure that includes the LSC (84261 bp) region, the SSC (13152 bp) region, and the IR (27868 bp) regions. A total of 131 genes were identified, including 86 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Comparative analysis of the genome revealed species variations in the LSC region associated with the geographical location of the V. amygdalina plant. Phylogenetic analysis of the chloroplast genomes using FastTree showed that V. amygdalina clustered closest to the chloroplast of the Crepidiastrum genus. This study identifies the characteristics of the V. amygdalina chloroplast genome, which will improve our understanding of intraspecies diversity and the identification of potential molecular markers to determine geographical origin.
Keywords
V. Amygdalina; Chloroplast genome; Medicinal Plant; Geographical location; Gene polymorphism.
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
