Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antioxidant Phytocomplexes Isolated from Pomegranate (Punica granatum L.) Using Hydrodynamic Cavitation Reveal Potential Application as Adjuvants in Cancer Therapies

Antonella Minutolo
† ORCID logo ,
Angelo Gismondi
† ORCID logo ,
Rossella Chirico
,
Gabriele Di Marco
ORCID logo ,
Vita Petrone
ORCID logo ,
Marialaura Fanelli
,
Alessia D'Agostino
,
Antonella Canini
ORCID logo ,
Sandro Grelli
ORCID logo ,
Lorenzo Albanese
,
Mauro Centritto
ORCID logo ,
Federica Zabini
,
Claudia Matteucci
ORCID logo ,
Francesco Meneguzzo
* ORCID logo
†
These authors contributed equally to this work.
Version 1 : Received: 10 July 2023 / Approved: 11 July 2023 / Online: 11 July 2023 (10:50:56 CEST)

A peer-reviewed article of this Preprint also exists.

Minutolo, A.; Gismondi, A.; Chirico, R.; Di Marco, G.; Petrone, V.; Fanelli, M.; D’Agostino, A.; Canini, A.; Grelli, S.; Albanese, L.; Centritto, M.; Zabini, F.; Matteucci, C.; Meneguzzo, F. Antioxidant Phytocomplexes Extracted from Pomegranate (Punica granatum L.) Using Hydrodynamic Cavitation Show Potential Anticancer Activity In Vitro. Antioxidants 2023, 12, 1560. Minutolo, A.; Gismondi, A.; Chirico, R.; Di Marco, G.; Petrone, V.; Fanelli, M.; D’Agostino, A.; Canini, A.; Grelli, S.; Albanese, L.; Centritto, M.; Zabini, F.; Matteucci, C.; Meneguzzo, F. Antioxidant Phytocomplexes Extracted from Pomegranate (Punica granatum L.) Using Hydrodynamic Cavitation Show Potential Anticancer Activity In Vitro. Antioxidants 2023, 12, 1560.

Abstract

The affordable production of valuable antioxidant extracts from plant resources depends on the availability of enabling technologies, such as hydrodynamic cavitation (HC), characterized by unrivalled effectiveness, efficiency, and scalability in the extraction of natural products. As sources of phytochemicals showing biological effects on human health, including anti-cancer properties, pomegranate (Punica granatum L.) fruits were processed using HC. Fractions collected during the process (M1-M5) were lyophilized (L), filtered (A), or used as such (C), for identifying their best conservation practice. Biochemical profile and in vitro antioxidant power were investi-gated by spectrophotometric and chromatographic assays. The antiproliferative effect of L-M3, resulting from just 15 minutes of extraction, was tested on human breast cancer line (AU565-PAR) and peripheral blood mononuclear (PBMCs) cells from healthy donors. M3-M4 fractions from L and C series revealed the highest antiradical activity and phenolic content. Cell growth, death, and redox state were assessed, showing that the pomegranate extract M3 significantly reduced tumor cell proliferation and intracellular oxygen reactive species. No effect on PBMCs was de-tected. Thus, the use of antioxidants isolated using HC as adjuvants in anticancer therapies might be advantageous and affordable; moreover, since oxidative stress contributes to cancer devel-opment, its reduction might be the leading mechanism.

Keywords

anti-cancer; antiradicals; bioactive metabolites; green extraction; hydrodynamic cavitation; phytochemicals; pomegranate

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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