Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Recent Developments on Colorimetric and Dual Colorimetric/Fluorimetric Enzymatic Biosensors for the Detection of Pesticides in Food

Version 1 : Received: 7 July 2023 / Approved: 7 July 2023 / Online: 10 July 2023 (11:12:40 CEST)

How to cite: Gaudin, V. Recent Developments on Colorimetric and Dual Colorimetric/Fluorimetric Enzymatic Biosensors for the Detection of Pesticides in Food. Preprints 2023, 2023070526. https://doi.org/10.20944/preprints202307.0526.v1 Gaudin, V. Recent Developments on Colorimetric and Dual Colorimetric/Fluorimetric Enzymatic Biosensors for the Detection of Pesticides in Food. Preprints 2023, 2023070526. https://doi.org/10.20944/preprints202307.0526.v1

Abstract

Pesticides are chemical products are used to protect crops to prevent or eliminate undesirable organisms. Pesticides can contaminate continental waters, air, soil and food products. The presence of pesticide residues must therefore be monitored in water and food using sensitive, reliable and broad-spectrum analytical methods, as there is a huge variety of pesticides. Biosensors are one of the screening methods with great potential for detecting residues below the maximum residue limits. The different sensing principles of enzymatic biosensors for pesticide analysis will be described. This review highlights the great potential of colorimetric, enzymatic biosensors for detecting pesticide residues in water and food. Moreover the development of dual colorimetric and fluorimetric sensors during the past years is presented. These methods are most often based on the enzyme acetylcholinesterase, whose activity is inhibited by pesticides. The different approaches used in the development of these biosensors will be detailed, based on different chemical substrates, fluorophores and nanomaterials. The advantages and disadvantages of each approach will be discussed. Finally, the development prospects for improving these biosensors will be presented.

Keywords

colorimetric; dual ratiometric; biosensor; enzymatic; acetylcholinesterase; plant and animal derived-food

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

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