In vitro study of bacterial adhesion and biofilm growth on fresh, frozen, or decellularized human bone grafts

Guillaume Villatte; Guillaume Fassot; Roger ERIVAN; Jean-Marie Nedelec; Stéphane Descamps; Yohann Wittrant; Stéphane BOISGARD; Ousmane Traore

doi:10.20944/preprints202307.0509.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 6 July 2023 / Approved: 7 July 2023 / Online: 10 July 2023 (03:40:37 CEST)

There was no significant difference in bacterial adhesion between the 3 bone graft types tested, regardless of the bacterial strain used (p > 0.22). After 72 hours of culture, there was no significant difference between the Fresh and Frozen bone groups, while the number of CFU in the Decellularised group was significantly lower (p < 0.01), averaging -0.72 +/- 0.08 lg, -0.20 +/- 0.01 lg and -0.78 +/- 0.48 lg with S. epidermidis, S. aureus and E. coli respectively. Absolute values differences are low, and considering fresh bone (equivalent to autograft) as the gold standard due to its low infection rates in clinical studies, we can conclude that adhesion and biolfilm development characteristics are similar between Fresh, Frozen and Decellularised bone grafts..

orthopaedic surgery, surgical site infection, bone graft, bacteria, S. aureus, biofilm, adhesion

Medicine and Pharmacology, Orthopedics and Sports Medicine

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In vitro study of bacterial adhesion and biofilm growth on fresh, frozen, or decellularized human bone grafts

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